<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2020 Workiva-->
<!--r:c64ea3b6-6c83-47d2-92d5-c468486547fb,g:0420881c-99c0-45a9-9d3a-19dde9b6dd82-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.ncino.com/role/Cover" xlink:type="simple" xlink:href="ncno-20201031.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/Cover" xlink:type="extended" id="i41e1d33c67e241c6a4b931579875cd38_Cover"/>
  <link:roleRef roleURI="http://www.ncino.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="ncno-20201031.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended" id="i73c6ec89e65747b7800b12b2e7c1c15b_CondensedConsolidatedBalanceSheets">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_4a34f0dd-1c8b-4450-9d14-a5a750c721a1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4a34f0dd-1c8b-4450-9d14-a5a750c721a1" xlink:to="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_ec39e930-1128-4d52-b3bd-02d0e84da123" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_ec39e930-1128-4d52-b3bd-02d0e84da123" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_6ff074d1-5dba-4fbb-bdb0-b5c9aa599425" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_6ff074d1-5dba-4fbb-bdb0-b5c9aa599425" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableRelatedPartiesCurrent_66eae083-f043-4434-aa54-329530523ef6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableRelatedPartiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_AccountsReceivableRelatedPartiesCurrent_66eae083-f043-4434-aa54-329530523ef6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetCurrent_da23c9a2-7272-47d8-9437-5151aebfc73e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalizedContractCostNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_CapitalizedContractCostNetCurrent_da23c9a2-7272-47d8-9437-5151aebfc73e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_a24c4f04-ac61-4a7a-bbf9-b78bc6fa7e30" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_a24c4f04-ac61-4a7a-bbf9-b78bc6fa7e30" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_06449ac3-c5dd-4248-be40-bc02962e3e88" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_4916d2e4-c864-4381-9fe9-fba09b7ad3fc" xlink:to="loc_us-gaap_AssetsCurrent_06449ac3-c5dd-4248-be40-bc02962e3e88" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_f11b828c-a106-4d3a-abc1-9b3236474049" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_f11b828c-a106-4d3a-abc1-9b3236474049" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetNoncurrent_61caa7e8-0325-4164-a25b-a6f66bbb8800" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalizedContractCostNetNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_CapitalizedContractCostNetNoncurrent_61caa7e8-0325-4164-a25b-a6f66bbb8800" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_6374ece5-54b3-4bc1-bb0c-6d3712b5c8b7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_Goodwill_6374ece5-54b3-4bc1-bb0c-6d3712b5c8b7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_171f6327-81ff-457b-86c9-8be0a770393a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_171f6327-81ff-457b-86c9-8be0a770393a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_c39c06c2-99a7-4bac-b543-4852acbd83ba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_c39c06c2-99a7-4bac-b543-4852acbd83ba" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_e12e510b-9d07-49b0-bf5f-f7f6ad659220" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_6bc9bec0-e9fa-4310-ae70-b6c32594f36f" xlink:to="loc_us-gaap_Assets_e12e510b-9d07-49b0-bf5f-f7f6ad659220" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4a34f0dd-1c8b-4450-9d14-a5a750c721a1" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_906ab823-eb07-49fd-ba29-32298535abd4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_AccountsPayableCurrent_906ab823-eb07-49fd-ba29-32298535abd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableRelatedPartiesCurrent_f0588cc6-5ea9-40f0-9f87-405320dc8ab7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsPayableRelatedPartiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_AccountsPayableRelatedPartiesCurrent_f0588cc6-5ea9-40f0-9f87-405320dc8ab7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedSalesCommissionCurrent_93aea56e-b7b1-4bbc-8ad0-36a772a01784" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccruedSalesCommissionCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_AccruedSalesCommissionCurrent_93aea56e-b7b1-4bbc-8ad0-36a772a01784" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_12f14016-fe0e-4396-b4c7-12824692f2f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_12f14016-fe0e-4396-b4c7-12824692f2f5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRentCreditCurrent_cb7daa04-4259-4554-8266-7f5e72c03634" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredRentCreditCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_DeferredRentCreditCurrent_cb7daa04-4259-4554-8266-7f5e72c03634" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_eab2d018-14b6-41ec-97b2-f6b3ee9ebe54" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_eab2d018-14b6-41ec-97b2-f6b3ee9ebe54" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_1c9362fd-548a-49d1-ac7a-6512daba9043" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_26e0a10a-6b37-4653-9729-6e5beef5a303" xlink:to="loc_us-gaap_LiabilitiesCurrent_1c9362fd-548a-49d1-ac7a-6512daba9043" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_ae5b6a91-c8a0-4672-be84-8ca65c4b81d9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_ae5b6a91-c8a0-4672-be84-8ca65c4b81d9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRentCreditNoncurrent_6d9fb21a-36a6-4970-b8f9-af37c233e560" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredRentCreditNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_DeferredRentCreditNoncurrent_6d9fb21a-36a6-4970-b8f9-af37c233e560" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_f95aaa3f-44e4-4aa5-960f-badccf54afc8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_f95aaa3f-44e4-4aa5-960f-badccf54afc8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_70df2f66-4e20-4ce6-b88e-4f48693c7ac5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_70df2f66-4e20-4ce6-b88e-4f48693c7ac5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_cdfdbf15-84e3-4e84-bd3c-2f2b47ba703d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_Liabilities_cdfdbf15-84e3-4e84-bd3c-2f2b47ba703d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_1eff0188-7081-4d66-a88b-f67d226735a1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_CommitmentsAndContingencies_1eff0188-7081-4d66-a88b-f67d226735a1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableNoncontrollingInterestEquityCarryingAmount_3bedb144-2626-4233-b732-29e8489c56f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RedeemableNoncontrollingInterestEquityCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_RedeemableNoncontrollingInterestEquityCarryingAmount_3bedb144-2626-4233-b732-29e8489c56f6" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_f13ad254-c435-433c-828d-3bf5814eee29" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_PreferredStockValue_f13ad254-c435-433c-828d-3bf5814eee29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_fd7f927b-cb15-445e-9708-d87ae61e6886" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_CommonStockValue_fd7f927b-cb15-445e-9708-d87ae61e6886" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_470335ef-4ceb-46b9-8d41-b884def7396e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_AdditionalPaidInCapital_470335ef-4ceb-46b9-8d41-b884def7396e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_ebf22aa0-abb3-4ce7-970b-ae0f974a2978" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_ebf22aa0-abb3-4ce7-970b-ae0f974a2978" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_a3633d95-1314-44e9-adc8-57f4dc8c1ff0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_a3633d95-1314-44e9-adc8-57f4dc8c1ff0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_11526df8-ca6d-4d01-846a-e71b27deb330" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterestAbstract_678d00f1-9f96-4b1e-932a-cb5d83383ca3" xlink:to="loc_us-gaap_StockholdersEquity_11526df8-ca6d-4d01-846a-e71b27deb330" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_069b1e45-9013-46c3-90b4-b5533a8958b5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_e952bf0c-3608-4e12-9a61-95acddec0f63" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_069b1e45-9013-46c3-90b4-b5533a8958b5" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_a06783ad-bdfa-4f42-92eb-d0af9032b08f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_4a34f0dd-1c8b-4450-9d14-a5a750c721a1" xlink:to="loc_us-gaap_StatementTable_a06783ad-bdfa-4f42-92eb-d0af9032b08f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_d863c601-0b5d-4827-9cb6-3a1ead601458" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_a06783ad-bdfa-4f42-92eb-d0af9032b08f" xlink:to="loc_us-gaap_StatementClassOfStockAxis_d863c601-0b5d-4827-9cb6-3a1ead601458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_d863c601-0b5d-4827-9cb6-3a1ead601458_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_d863c601-0b5d-4827-9cb6-3a1ead601458" xlink:to="loc_us-gaap_ClassOfStockDomain_d863c601-0b5d-4827-9cb6-3a1ead601458_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_47847d08-029d-4c6d-a91f-48afec2897ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_d863c601-0b5d-4827-9cb6-3a1ead601458" xlink:to="loc_us-gaap_ClassOfStockDomain_47847d08-029d-4c6d-a91f-48afec2897ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VotingCommonStockMember_e5025ec4-b1b3-4e2a-a41d-48911a655dfb" xlink:href="ncno-20201031.xsd#ncno_VotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_47847d08-029d-4c6d-a91f-48afec2897ed" xlink:to="loc_ncno_VotingCommonStockMember_e5025ec4-b1b3-4e2a-a41d-48911a655dfb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonvotingCommonStockMember_a5254e1f-8fda-4531-a385-118e111b9046" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonvotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_47847d08-029d-4c6d-a91f-48afec2897ed" xlink:to="loc_us-gaap_NonvotingCommonStockMember_a5254e1f-8fda-4531-a385-118e111b9046" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f500dbcc-dcc0-4136-acab-84c911f751e7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_a06783ad-bdfa-4f42-92eb-d0af9032b08f" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f500dbcc-dcc0-4136-acab-84c911f751e7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_f500dbcc-dcc0-4136-acab-84c911f751e7_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f500dbcc-dcc0-4136-acab-84c911f751e7" xlink:to="loc_us-gaap_RelatedPartyDomain_f500dbcc-dcc0-4136-acab-84c911f751e7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_ae187720-d866-4f28-a741-2a62f6a62b73" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f500dbcc-dcc0-4136-acab-84c911f751e7" xlink:to="loc_us-gaap_RelatedPartyDomain_ae187720-d866-4f28-a741-2a62f6a62b73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_b4259ff8-69ca-4025-9847-ce0e25eb7834" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_ae187720-d866-4f28-a741-2a62f6a62b73" xlink:to="loc_srt_AffiliatedEntityMember_b4259ff8-69ca-4025-9847-ce0e25eb7834" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="ncno-20201031.xsd#CondensedConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="extended" id="i08d12381c28d485abe72ca4cff9edb53_CondensedConsolidatedBalanceSheetsParenthetical">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_29d8c281-b447-4f0d-b2b5-4ff974871cc4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_29d8c281-b447-4f0d-b2b5-4ff974871cc4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent_3e58b364-50e5-4997-adad-60497ed77bba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableCurrent_3e58b364-50e5-4997-adad-60497ed77bba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0a4a2e20-5987-4881-be88-84f3338cac1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0a4a2e20-5987-4881-be88-84f3338cac1b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_45f239d2-2707-445d-be3f-cc0ce674299d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_45f239d2-2707-445d-be3f-cc0ce674299d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_a0239bc0-c4ed-4c06-b0b9-2aea0254c127" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_PreferredStockSharesIssued_a0239bc0-c4ed-4c06-b0b9-2aea0254c127" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_53690f06-cb4f-4cdf-9343-aa3e077812d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_53690f06-cb4f-4cdf-9343-aa3e077812d2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_565153a3-45e2-4013-b726-1aa36b7fa97d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_565153a3-45e2-4013-b726-1aa36b7fa97d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_226854c3-f1ab-430d-ba2d-d3d650e42955" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_226854c3-f1ab-430d-ba2d-d3d650e42955" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_54a2611c-ada8-4324-bc39-251f4e06fe33" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_CommonStockSharesIssued_54a2611c-ada8-4324-bc39-251f4e06fe33" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_e7032ae0-fe6c-40ec-a0f9-5ab81be37d9b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_e7032ae0-fe6c-40ec-a0f9-5ab81be37d9b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_7d2a42cf-fbde-4e4b-a984-4f3db308f96c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_c74484b3-baff-4fc9-9908-79a1fb95c832" xlink:to="loc_us-gaap_StatementTable_7d2a42cf-fbde-4e4b-a984-4f3db308f96c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7d2a42cf-fbde-4e4b-a984-4f3db308f96c" xlink:to="loc_srt_ConsolidatedEntitiesAxis_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee" xlink:to="loc_srt_ConsolidatedEntitiesDomain_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_bfc537af-ca03-46e3-9ac6-9ccfa24634be" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_ffeb2c5e-78ba-4abc-8165-0ffa683c6cee" xlink:to="loc_srt_ConsolidatedEntitiesDomain_bfc537af-ca03-46e3-9ac6-9ccfa24634be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableInterestEntityPrimaryBeneficiaryMember_a7b4fd49-8ce0-4606-ba2a-529b82926027" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableInterestEntityPrimaryBeneficiaryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_bfc537af-ca03-46e3-9ac6-9ccfa24634be" xlink:to="loc_us-gaap_VariableInterestEntityPrimaryBeneficiaryMember_a7b4fd49-8ce0-4606-ba2a-529b82926027" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7d2a42cf-fbde-4e4b-a984-4f3db308f96c" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f" xlink:to="loc_us-gaap_ClassOfStockDomain_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e4ddb265-05e9-4938-a2db-83254e8bf273" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b169c7ec-736a-47cf-88e6-c5c2e8cddf8f" xlink:to="loc_us-gaap_ClassOfStockDomain_e4ddb265-05e9-4938-a2db-83254e8bf273" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VotingCommonStockMember_c07e1fd0-c621-4852-babc-23b1ac275f8b" xlink:href="ncno-20201031.xsd#ncno_VotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_e4ddb265-05e9-4938-a2db-83254e8bf273" xlink:to="loc_ncno_VotingCommonStockMember_c07e1fd0-c621-4852-babc-23b1ac275f8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonvotingCommonStockMember_1a2ba344-87e7-4f13-9e6e-f429c05eb03f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonvotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_e4ddb265-05e9-4938-a2db-83254e8bf273" xlink:to="loc_us-gaap_NonvotingCommonStockMember_1a2ba344-87e7-4f13-9e6e-f429c05eb03f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/ConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="ncno-20201031.xsd#ConsolidatedStatementsofOperations"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ConsolidatedStatementsofOperations" xlink:type="extended" id="iee873bd1337e4b44aa11f9145c963205_ConsolidatedStatementsofOperations">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_a0233dee-dc6b-4349-a426-45bd94433cc9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_RevenuesAbstract_a0233dee-dc6b-4349-a426-45bd94433cc9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_4eb7c3e3-5091-4543-937c-3b2f26382cbd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_a0233dee-dc6b-4349-a426-45bd94433cc9" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_4eb7c3e3-5091-4543-937c-3b2f26382cbd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfRevenueAbstract_e3aa121e-c1ad-4af7-a64a-ac4d529910b1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfRevenueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_CostOfRevenueAbstract_e3aa121e-c1ad-4af7-a64a-ac4d529910b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_bf67f86f-ec5b-462e-8424-4970837e649d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostOfRevenueAbstract_e3aa121e-c1ad-4af7-a64a-ac4d529910b1" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_bf67f86f-ec5b-462e-8424-4970837e649d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_2760d398-3b59-41c3-8312-68b6a60aaf2e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_GrossProfit_2760d398-3b59-41c3-8312-68b6a60aaf2e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpense_75141a1e-c4dc-4b60-afe5-0d976eedb350" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:to="loc_us-gaap_SellingAndMarketingExpense_75141a1e-c4dc-4b60-afe5-0d976eedb350" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_77c62be9-b053-41d5-aa08-8f20743e7530" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_77c62be9-b053-41d5-aa08-8f20743e7530" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_7a0c937e-c27f-47b0-bb82-2669d8c7ae2a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_7a0c937e-c27f-47b0-bb82-2669d8c7ae2a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_faf6f5ce-d37c-4ea4-85f3-3fe47436f724" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_d4e0fa52-4504-4933-8fbb-e818416b9602" xlink:to="loc_us-gaap_OperatingExpenses_faf6f5ce-d37c-4ea4-85f3-3fe47436f724" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_b3a4cdb8-2d68-4919-9771-f909651e8b3a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_OperatingIncomeLoss_b3a4cdb8-2d68-4919-9771-f909651e8b3a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpenseAbstract_291ab3da-c4c9-47aa-91f4-e1a8f2e56e96" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonoperatingIncomeExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_NonoperatingIncomeExpenseAbstract_291ab3da-c4c9-47aa-91f4-e1a8f2e56e96" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_73b8fea2-c113-4bf8-9140-133106214505" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_291ab3da-c4c9-47aa-91f4-e1a8f2e56e96" xlink:to="loc_us-gaap_InvestmentIncomeInterest_73b8fea2-c113-4bf8-9140-133106214505" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_0dcae489-dd97-45af-a102-fa24c5eb996e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_291ab3da-c4c9-47aa-91f4-e1a8f2e56e96" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_0dcae489-dd97-45af-a102-fa24c5eb996e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossIncludingPortionAttributableToNoncontrollingInterest_34a6b8a7-c53d-4f8d-a67b-55d19ce7fda9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeLossIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_IncomeLossIncludingPortionAttributableToNoncontrollingInterest_34a6b8a7-c53d-4f8d-a67b-55d19ce7fda9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_4778fdec-9922-40e8-a1cf-50b022810e62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_4778fdec-9922-40e8-a1cf-50b022810e62" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_b55cc1bf-9df3-401c-be43-42cd9784031d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_ProfitLoss_b55cc1bf-9df3-401c-be43-42cd9784031d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAttributableToRedeemableNoncontrollingInterest_0c1fd630-ee41-437b-8013-a62742caaec5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLossAttributableToRedeemableNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_NetIncomeLossAttributableToRedeemableNoncontrollingInterest_0c1fd630-ee41-437b-8013-a62742caaec5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_NetIncomeLossAdjustmentsToRedeemableNoncontrollingInterest_fc40cc89-67f8-41cf-af8f-27b55cf260cd" xlink:href="ncno-20201031.xsd#ncno_NetIncomeLossAdjustmentsToRedeemableNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_ncno_NetIncomeLossAdjustmentsToRedeemableNoncontrollingInterest_fc40cc89-67f8-41cf-af8f-27b55cf260cd" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_645af378-2086-4f86-aa46-ab5cc9907ca5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_NetIncomeLoss_645af378-2086-4f86-aa46-ab5cc9907ca5" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareAbstract_0ca9d42e-7d21-4f43-aa4b-a960a99fa874" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EarningsPerShareAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_EarningsPerShareAbstract_0ca9d42e-7d21-4f43-aa4b-a960a99fa874" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasicAndDiluted_718d75f3-5f2f-4c6c-b382-02db587e550c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EarningsPerShareBasicAndDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_0ca9d42e-7d21-4f43-aa4b-a960a99fa874" xlink:to="loc_us-gaap_EarningsPerShareBasicAndDiluted_718d75f3-5f2f-4c6c-b382-02db587e550c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_WeightedAverageNumberOfSharesOutstandingBasicAndDilutedAbstract_eae64a5d-d2b5-40ef-bbfd-b35c7d14c5f0" xlink:href="ncno-20201031.xsd#ncno_WeightedAverageNumberOfSharesOutstandingBasicAndDilutedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_ncno_WeightedAverageNumberOfSharesOutstandingBasicAndDilutedAbstract_eae64a5d-d2b5-40ef-bbfd-b35c7d14c5f0" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted_fbf98f1d-3c25-43a9-8dc1-61d863d89179" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ncno_WeightedAverageNumberOfSharesOutstandingBasicAndDilutedAbstract_eae64a5d-d2b5-40ef-bbfd-b35c7d14c5f0" xlink:to="loc_us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted_fbf98f1d-3c25-43a9-8dc1-61d863d89179" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_19b6eb35-0fee-4ce0-9834-3a902e8881f2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_909bbc59-32fb-4c1f-8d47-0da92f732a14" xlink:to="loc_us-gaap_StatementTable_19b6eb35-0fee-4ce0-9834-3a902e8881f2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_3279f9a1-94af-4fde-8e0a-f18910461ccd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_19b6eb35-0fee-4ce0-9834-3a902e8881f2" xlink:to="loc_srt_ProductOrServiceAxis_3279f9a1-94af-4fde-8e0a-f18910461ccd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_3279f9a1-94af-4fde-8e0a-f18910461ccd_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_3279f9a1-94af-4fde-8e0a-f18910461ccd" xlink:to="loc_srt_ProductsAndServicesDomain_3279f9a1-94af-4fde-8e0a-f18910461ccd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_33a484b9-8b0d-4080-9b9c-fc9ce0f71dc3" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_3279f9a1-94af-4fde-8e0a-f18910461ccd" xlink:to="loc_srt_ProductsAndServicesDomain_33a484b9-8b0d-4080-9b9c-fc9ce0f71dc3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_21805b4f-4aed-4868-acc7-cfcdbca70345" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_33a484b9-8b0d-4080-9b9c-fc9ce0f71dc3" xlink:to="loc_us-gaap_LicenseAndServiceMember_21805b4f-4aed-4868-acc7-cfcdbca70345" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ProfessionalServicesMember_c12d96bf-6b21-448d-b71d-a0129b29ba59" xlink:href="ncno-20201031.xsd#ncno_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_33a484b9-8b0d-4080-9b9c-fc9ce0f71dc3" xlink:to="loc_ncno_ProfessionalServicesMember_c12d96bf-6b21-448d-b71d-a0129b29ba59" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/ConsolidatedStatementsofOperationsParenthetical" xlink:type="simple" xlink:href="ncno-20201031.xsd#ConsolidatedStatementsofOperationsParenthetical"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ConsolidatedStatementsofOperationsParenthetical" xlink:type="extended" id="i0910fa6858344beaab8756e00fc4e438_ConsolidatedStatementsofOperationsParenthetical">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_84e75973-eaab-493b-ab24-838ca2bc6422" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromRelatedParties_9d8f34f3-8186-4549-967f-71b9defdec1a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_84e75973-eaab-493b-ab24-838ca2bc6422" xlink:to="loc_us-gaap_RevenueFromRelatedParties_9d8f34f3-8186-4549-967f-71b9defdec1a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyCosts_38ce8e38-f365-4f56-a0a4-641752289f28" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_84e75973-eaab-493b-ab24-838ca2bc6422" xlink:to="loc_us-gaap_RelatedPartyCosts_38ce8e38-f365-4f56-a0a4-641752289f28" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_81346bc5-eb04-4345-9334-b01d2610eb1a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_84e75973-eaab-493b-ab24-838ca2bc6422" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_81346bc5-eb04-4345-9334-b01d2610eb1a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_e876d551-093d-4451-a0ef-4be7c805799d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_84e75973-eaab-493b-ab24-838ca2bc6422" xlink:to="loc_us-gaap_StatementTable_e876d551-093d-4451-a0ef-4be7c805799d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_d0fbde7e-a766-426b-8b22-abc79d073929" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_e876d551-093d-4451-a0ef-4be7c805799d" xlink:to="loc_srt_ProductOrServiceAxis_d0fbde7e-a766-426b-8b22-abc79d073929" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_d0fbde7e-a766-426b-8b22-abc79d073929_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_d0fbde7e-a766-426b-8b22-abc79d073929" xlink:to="loc_srt_ProductsAndServicesDomain_d0fbde7e-a766-426b-8b22-abc79d073929_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_19f0a20c-f2c8-45af-a85d-31b6a6c0baf7" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_d0fbde7e-a766-426b-8b22-abc79d073929" xlink:to="loc_srt_ProductsAndServicesDomain_19f0a20c-f2c8-45af-a85d-31b6a6c0baf7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAndServiceMember_691ef68d-a7f5-426b-81c1-79365f9d4f43" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LicenseAndServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_19f0a20c-f2c8-45af-a85d-31b6a6c0baf7" xlink:to="loc_us-gaap_LicenseAndServiceMember_691ef68d-a7f5-426b-81c1-79365f9d4f43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ProfessionalServicesMember_5cc2cffc-0bf4-497d-bc59-7cd9ba213138" xlink:href="ncno-20201031.xsd#ncno_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_19f0a20c-f2c8-45af-a85d-31b6a6c0baf7" xlink:to="loc_ncno_ProfessionalServicesMember_5cc2cffc-0bf4-497d-bc59-7cd9ba213138" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_d3301223-00fb-486f-839e-d607d0ace927" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_e876d551-093d-4451-a0ef-4be7c805799d" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_d3301223-00fb-486f-839e-d607d0ace927" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_d3301223-00fb-486f-839e-d607d0ace927_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d3301223-00fb-486f-839e-d607d0ace927" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_d3301223-00fb-486f-839e-d607d0ace927_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d3301223-00fb-486f-839e-d607d0ace927" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_1c7c6dac-a585-409c-914f-d8c5234004ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:to="loc_us-gaap_CostOfSalesMember_1c7c6dac-a585-409c-914f-d8c5234004ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_5e98a1d6-dfef-4bfc-a69f-8224a3e497ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_5e98a1d6-dfef-4bfc-a69f-8224a3e497ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_4698a722-3046-4d4f-9fbb-2628e6246886" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_4698a722-3046-4d4f-9fbb-2628e6246886" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_68005b76-8b51-4925-bc05-7facf998f2f3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_5139ad69-df9a-43ce-98d7-373f133da46f" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_68005b76-8b51-4925-bc05-7facf998f2f3" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/ConsolidatedStatementofComprehensiveLossUnaudited" xlink:type="simple" xlink:href="ncno-20201031.xsd#ConsolidatedStatementofComprehensiveLossUnaudited"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ConsolidatedStatementofComprehensiveLossUnaudited" xlink:type="extended" id="idcac94f7566f402d808b2a5046c360d2_ConsolidatedStatementofComprehensiveLossUnaudited"/>
  <link:roleRef roleURI="http://www.ncino.com/role/UnauditedConsolidatedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="ncno-20201031.xsd#UnauditedConsolidatedStatementsofStockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/UnauditedConsolidatedStatementsofStockholdersEquity" xlink:type="extended" id="i8abc33acfc2e4a64a768c5ee1f9042ea_UnauditedConsolidatedStatementsofStockholdersEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_45536856-598a-49d2-ab30-f30f51f5c519" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_45536856-598a-49d2-ab30-f30f51f5c519" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_b6d7e45a-6050-4817-8bf5-321d8d45ffb3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_SharesOutstanding_b6d7e45a-6050-4817-8bf5-321d8d45ffb3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_e97bfab2-1f6c-4698-b201-dc18fb6c77f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_e97bfab2-1f6c-4698-b201-dc18fb6c77f1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_18f9bb88-ccaf-48fe-8b75-be304aedca8c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_18f9bb88-ccaf-48fe-8b75-be304aedca8c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_b93982fa-bc21-4f65-ad35-e0badd048194" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_b93982fa-bc21-4f65-ad35-e0badd048194" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_45854d65-3fa8-44a6-b592-bb0f86a8ee32" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_45854d65-3fa8-44a6-b592-bb0f86a8ee32" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_4b8add3c-225b-4cec-b72a-297cee441e7e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_4b8add3c-225b-4cec-b72a-297cee441e7e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_9ddc1a07-d164-4817-8d86-b1b4f4537959" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_9ddc1a07-d164-4817-8d86-b1b4f4537959" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ContingentConsiderationRelatedToBusinessCombinations_554d73e9-b793-4132-b99a-ab4aac2cfa9b" xlink:href="ncno-20201031.xsd#ncno_ContingentConsiderationRelatedToBusinessCombinations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_ncno_ContingentConsiderationRelatedToBusinessCombinations_554d73e9-b793-4132-b99a-ab4aac2cfa9b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_7da2f567-8dc1-421d-8580-1cc1e3595469" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_7da2f567-8dc1-421d-8580-1cc1e3595469" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_3e9e7f8e-c351-4b16-a489-b02cc0e6d446" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_3e9e7f8e-c351-4b16-a489-b02cc0e6d446" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_StockIssuedDuringPeriodSharesReclassificationOfCommonStock_03926e23-92de-465a-ad16-70801a8d1b23" xlink:href="ncno-20201031.xsd#ncno_StockIssuedDuringPeriodSharesReclassificationOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_ncno_StockIssuedDuringPeriodSharesReclassificationOfCommonStock_03926e23-92de-465a-ad16-70801a8d1b23" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_StockIssuedDuringPeriodValueReclassificationOfCommonStock_74c95964-bc44-4fef-90f1-d3ce5e1dc247" xlink:href="ncno-20201031.xsd#ncno_StockIssuedDuringPeriodValueReclassificationOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_ncno_StockIssuedDuringPeriodValueReclassificationOfCommonStock_74c95964-bc44-4fef-90f1-d3ce5e1dc247" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_01f0629a-2244-442b-acce-6d8501602c46" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_01f0629a-2244-442b-acce-6d8501602c46" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_023c6a48-b9fd-43c3-8175-44f58fb8b9cf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_023c6a48-b9fd-43c3-8175-44f58fb8b9cf" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_31248c5a-b01b-4b4d-a9a7-083f29c7bbc1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_3b18b23e-cb09-4c99-8b99-9b1b455b2d46" xlink:to="loc_us-gaap_NetIncomeLoss_31248c5a-b01b-4b4d-a9a7-083f29c7bbc1" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_9f5b9d25-bfde-4857-a771-fe0defb581f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_3c4a0347-2bb4-4a98-b3f2-84e2e4702d5d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_4a2b1761-60b7-4bfe-966a-83a8713b3b36" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_45536856-598a-49d2-ab30-f30f51f5c519" xlink:to="loc_us-gaap_StatementTable_4a2b1761-60b7-4bfe-966a-83a8713b3b36" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_1019069c-6ab3-41c9-9322-b960089c1582" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_4a2b1761-60b7-4bfe-966a-83a8713b3b36" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_1019069c-6ab3-41c9-9322-b960089c1582" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_1019069c-6ab3-41c9-9322-b960089c1582_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_1019069c-6ab3-41c9-9322-b960089c1582" xlink:to="loc_us-gaap_EquityComponentDomain_1019069c-6ab3-41c9-9322-b960089c1582_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_1019069c-6ab3-41c9-9322-b960089c1582" xlink:to="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_af37983b-eeaa-4dca-ab2f-1430ae4c42d1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:to="loc_us-gaap_CommonStockMember_af37983b-eeaa-4dca-ab2f-1430ae4c42d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_f6215267-10ec-4111-b742-1851c4d56ee7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_f6215267-10ec-4111-b742-1851c4d56ee7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_03919bf7-44e7-4ea2-b2ea-a2a394abd594" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:to="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_03919bf7-44e7-4ea2-b2ea-a2a394abd594" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_73f3246b-b398-4e62-a65a-5ba0fc4da5fc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_35663d38-8a01-4eea-8d27-81ffa425efc1" xlink:to="loc_us-gaap_RetainedEarningsMember_73f3246b-b398-4e62-a65a-5ba0fc4da5fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_142eb498-ffa0-49d0-9eab-660f68cd5c2c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_4a2b1761-60b7-4bfe-966a-83a8713b3b36" xlink:to="loc_us-gaap_StatementClassOfStockAxis_142eb498-ffa0-49d0-9eab-660f68cd5c2c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_142eb498-ffa0-49d0-9eab-660f68cd5c2c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_142eb498-ffa0-49d0-9eab-660f68cd5c2c" xlink:to="loc_us-gaap_ClassOfStockDomain_142eb498-ffa0-49d0-9eab-660f68cd5c2c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_59bacabf-0ed1-4237-b4be-97580b2018c0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_142eb498-ffa0-49d0-9eab-660f68cd5c2c" xlink:to="loc_us-gaap_ClassOfStockDomain_59bacabf-0ed1-4237-b4be-97580b2018c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VotingCommonStockMember_368f4265-6e4e-485f-99ad-83b0faa0dcc9" xlink:href="ncno-20201031.xsd#ncno_VotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_59bacabf-0ed1-4237-b4be-97580b2018c0" xlink:to="loc_ncno_VotingCommonStockMember_368f4265-6e4e-485f-99ad-83b0faa0dcc9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonvotingCommonStockMember_68c17eea-805e-46b8-b7b0-657cc484ec0b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonvotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_59bacabf-0ed1-4237-b4be-97580b2018c0" xlink:to="loc_us-gaap_NonvotingCommonStockMember_68c17eea-805e-46b8-b7b0-657cc484ec0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_4a2b1761-60b7-4bfe-966a-83a8713b3b36" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_bef5a4c4-fde6-4f11-9447-310d882b2e59" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_dd0aa61d-af17-4518-abd8-6d68ec2c9bcf" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_bef5a4c4-fde6-4f11-9447-310d882b2e59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_af0f8944-c331-441d-a90d-b5c81e1f8627" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_bef5a4c4-fde6-4f11-9447-310d882b2e59" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_af0f8944-c331-441d-a90d-b5c81e1f8627" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/ConsolidatedStatementsofStockholdersEquityParenthetical" xlink:type="simple" xlink:href="ncno-20201031.xsd#ConsolidatedStatementsofStockholdersEquityParenthetical"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ConsolidatedStatementsofStockholdersEquityParenthetical" xlink:type="extended" id="i6dacad2c31b24f67be663c8bfd6c00ce_ConsolidatedStatementsofStockholdersEquityParenthetical"/>
  <link:roleRef roleURI="http://www.ncino.com/role/UnauditedStatementofCashFlowsStatement" xlink:type="simple" xlink:href="ncno-20201031.xsd#UnauditedStatementofCashFlowsStatement"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/UnauditedStatementofCashFlowsStatement" xlink:type="extended" id="iec09986dc37e4ec28b31bfe13797548b_UnauditedStatementofCashFlowsStatement">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_bd673736-0a2b-4579-9b52-a6b9b9099712" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_bd673736-0a2b-4579-9b52-a6b9b9099712" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c24acd0a-03d8-487d-a5bc-2a46763aeb13" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_bd673736-0a2b-4579-9b52-a6b9b9099712" xlink:to="loc_us-gaap_NetIncomeLoss_c24acd0a-03d8-487d-a5bc-2a46763aeb13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_NetIncomeLossAttributableToRedeemableNoncontrollingInterestNetOfAdjustment_fe8bc714-f39c-44fc-9f77-7103aac6772f" xlink:href="ncno-20201031.xsd#ncno_NetIncomeLossAttributableToRedeemableNoncontrollingInterestNetOfAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_bd673736-0a2b-4579-9b52-a6b9b9099712" xlink:to="loc_ncno_NetIncomeLossAttributableToRedeemableNoncontrollingInterestNetOfAdjustment_fe8bc714-f39c-44fc-9f77-7103aac6772f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_7292bccc-660b-4492-b78d-ca0cbfb0977b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_bd673736-0a2b-4579-9b52-a6b9b9099712" xlink:to="loc_us-gaap_ProfitLoss_7292bccc-660b-4492-b78d-ca0cbfb0977b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_a4495de8-2e2e-4847-8d3e-a7ba3c9334c9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_a4495de8-2e2e-4847-8d3e-a7ba3c9334c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostAmortization_a70d875b-8bfd-430c-a731-e54f63393c1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalizedContractCostAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:to="loc_us-gaap_CapitalizedContractCostAmortization_a70d875b-8bfd-430c-a731-e54f63393c1b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_3f13c4ed-cc1d-4d4c-9d28-5a3d4a29edf2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:to="loc_us-gaap_ShareBasedCompensation_3f13c4ed-cc1d-4d4c-9d28-5a3d4a29edf2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_df7347c9-7892-48fd-ba18-07e205c5af97" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_df7347c9-7892-48fd-ba18-07e205c5af97" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_ad507ca2-b9bc-4ce4-8325-4adc333e4540" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_73da9597-2468-4809-9518-386154922bbc" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_ad507ca2-b9bc-4ce4-8325-4adc333e4540" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInOperatingCapitalAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_de1fa111-8d34-497d-9a4e-1d627662445f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_de1fa111-8d34-497d-9a4e-1d627662445f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivableRelatedParties_a0f5f17e-7366-41ce-b439-28df1f24fefd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivableRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivableRelatedParties_a0f5f17e-7366-41ce-b439-28df1f24fefd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_IncreaseDecreaseInCostsToObtainContracts_55034b59-da19-43f5-8f27-9f2f6beeb454" xlink:href="ncno-20201031.xsd#ncno_IncreaseDecreaseInCostsToObtainContracts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_ncno_IncreaseDecreaseInCostsToObtainContracts_55034b59-da19-43f5-8f27-9f2f6beeb454" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_7dcbf0b5-78ed-45cb-bd6c-af62899e81b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_7dcbf0b5-78ed-45cb-bd6c-af62899e81b9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities_1fdef582-d196-454b-a3a6-f52727b02658" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities_1fdef582-d196-454b-a3a6-f52727b02658" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties_060db05f-9314-4ac6-b819-4edc74c36bf5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties_060db05f-9314-4ac6-b819-4edc74c36bf5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_IncreaseDecreaseInDeferredRent_adfe6b19-6215-4338-bbc1-4b01c6d7e8d2" xlink:href="ncno-20201031.xsd#ncno_IncreaseDecreaseInDeferredRent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_ncno_IncreaseDecreaseInDeferredRent_adfe6b19-6215-4338-bbc1-4b01c6d7e8d2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_759f6c45-bc63-4107-adfd-49cb226c69a2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_759f6c45-bc63-4107-adfd-49cb226c69a2" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_IncreaseDecreaseInContractWithCustomerLiabilityRelatedParties_88d133be-5fd1-44e3-a09c-3bb13f23aab1" xlink:href="ncno-20201031.xsd#ncno_IncreaseDecreaseInContractWithCustomerLiabilityRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_e9d49a26-dc01-4f44-908f-106b88fe80fe" xlink:to="loc_ncno_IncreaseDecreaseInContractWithCustomerLiabilityRelatedParties_88d133be-5fd1-44e3-a09c-3bb13f23aab1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_77e3ec69-32b1-4312-b91f-6fbcbc136630" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_77e3ec69-32b1-4312-b91f-6fbcbc136630" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_497f8d1b-bf90-4a89-9948-554a9a0646d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_497f8d1b-bf90-4a89-9948-554a9a0646d7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_bedfbcfa-274a-4a8e-9aaa-894f4ed70d79" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_497f8d1b-bf90-4a89-9948-554a9a0646d7" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_bedfbcfa-274a-4a8e-9aaa-894f4ed70d79" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_38d46410-f8a0-4103-b71b-1727388a2673" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_497f8d1b-bf90-4a89-9948-554a9a0646d7" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_38d46410-f8a0-4103-b71b-1727388a2673" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_7b0a236c-e5c2-47ce-928a-1ec02e2d9980" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_497f8d1b-bf90-4a89-9948-554a9a0646d7" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_7b0a236c-e5c2-47ce-928a-1ec02e2d9980" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_5720d6f6-0df3-47f1-82d2-9939eb67bce3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromIssuanceInitialPublicOffering"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_5720d6f6-0df3-47f1-82d2-9939eb67bce3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_52c0b0e0-4584-4fe5-916d-f8e26720d7d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_PaymentsOfStockIssuanceCosts_52c0b0e0-4584-4fe5-916d-f8e26720d7d2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_f7b90ad8-c892-4bb9-ae0a-ecf1656cb19f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMinorityShareholders_cd372893-9994-4c73-ab91-b204d6bb1776" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromMinorityShareholders"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_ProceedsFromMinorityShareholders_cd372893-9994-4c73-ab91-b204d6bb1776" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_6cede489-ce13-417b-901f-93e32442917d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_6cede489-ce13-417b-901f-93e32442917d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_PaymentsOfDeferredCosts_a9b59a78-9319-4158-abd3-966c63f66015" xlink:href="ncno-20201031.xsd#ncno_PaymentsOfDeferredCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_ncno_PaymentsOfDeferredCosts_a9b59a78-9319-4158-abd3-966c63f66015" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_2645de43-1134-4b05-af16-8c730581dfe5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_2645de43-1134-4b05-af16-8c730581dfe5" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_79b4e6c2-aacf-4ea9-859d-777b0114e822" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_61719f88-f672-4ce3-ae54-e6e233d8c8ec" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_79b4e6c2-aacf-4ea9-859d-777b0114e822" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents_bf105eac-c0fa-48fc-97b1-ae94ef29d0fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents_bf105eac-c0fa-48fc-97b1-ae94ef29d0fd" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_8f236c20-a29b-4d1a-a74d-55aa8af8843d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_8f236c20-a29b-4d1a-a74d-55aa8af8843d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_6617d479-7216-4d98-a914-a4d172b6713f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_6617d479-7216-4d98-a914-a4d172b6713f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_b3165200-73cf-483c-87e0-5e928734444c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_2303c4c9-3eb1-4ee5-a33d-56cac1ec6ca7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_2303c4c9-3eb1-4ee5-a33d-56cac1ec6ca7" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxesPaidNet_047a21a6-45b5-40f9-b250-be1182a2185e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxesPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_2303c4c9-3eb1-4ee5-a33d-56cac1ec6ca7" xlink:to="loc_us-gaap_IncomeTaxesPaidNet_047a21a6-45b5-40f9-b250-be1182a2185e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalExpendituresIncurredButNotYetPaid_49fff62d-966b-41bd-8a6b-8f2ace2c2602" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalExpendituresIncurredButNotYetPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_us-gaap_CapitalExpendituresIncurredButNotYetPaid_49fff62d-966b-41bd-8a6b-8f2ace2c2602" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssued1_9544292d-8137-41da-9394-cb93d29eadbd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssued1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_us-gaap_StockIssued1_9544292d-8137-41da-9394-cb93d29eadbd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_CostAccruedRelatedToInitialPublicOffering_c1b30dd9-6ac3-4f1c-bc8d-7eb331170910" xlink:href="ncno-20201031.xsd#ncno_CostAccruedRelatedToInitialPublicOffering"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_ncno_CostAccruedRelatedToInitialPublicOffering_c1b30dd9-6ac3-4f1c-bc8d-7eb331170910" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_CostAccruedRelatedToInitialPublicOfferingReclassifiedFromOtherLongTermAssetsToEquity_1d04d912-185d-4c81-8b7e-94fa570a9adc" xlink:href="ncno-20201031.xsd#ncno_CostAccruedRelatedToInitialPublicOfferingReclassifiedFromOtherLongTermAssetsToEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_ncno_CostAccruedRelatedToInitialPublicOfferingReclassifiedFromOtherLongTermAssetsToEquity_1d04d912-185d-4c81-8b7e-94fa570a9adc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsInOtherLongTermLiabilities_af688c61-d481-45b4-aa5c-fbe63e57d605" xlink:href="ncno-20201031.xsd#ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsInOtherLongTermLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsInOtherLongTermLiabilities_af688c61-d481-45b4-aa5c-fbe63e57d605" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsIncludedInEquity_5e6c78cb-c355-45f0-abdf-1f634204857e" xlink:href="ncno-20201031.xsd#ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsIncludedInEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNoncashInvestingAndFinancingItemsAbstract_8bd721c0-135f-4dd8-9244-7b77d6703407" xlink:to="loc_ncno_FairValueOfContingentConsiderationInConnectionWithBusinessAcquisitionsIncludedInEquity_5e6c78cb-c355-45f0-abdf-1f634204857e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_7f44beb3-a705-4c2a-a6aa-60f276ca5268" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_e0a233b6-d1eb-4c5e-ae74-2839139d091b" xlink:to="loc_us-gaap_StatementTable_7f44beb3-a705-4c2a-a6aa-60f276ca5268" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_ffef74fb-c734-4729-9891-675a5b2f03bc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7f44beb3-a705-4c2a-a6aa-60f276ca5268" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_ffef74fb-c734-4729-9891-675a5b2f03bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ffef74fb-c734-4729-9891-675a5b2f03bc_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ffef74fb-c734-4729-9891-675a5b2f03bc" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ffef74fb-c734-4729-9891-675a5b2f03bc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_3d5c9e3a-f181-4612-82fe-821f99a363ba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ffef74fb-c734-4729-9891-675a5b2f03bc" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_3d5c9e3a-f181-4612-82fe-821f99a363ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IPOMember_72652af5-fe50-411b-8ac2-eea4bb116bb0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IPOMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_3d5c9e3a-f181-4612-82fe-821f99a363ba" xlink:to="loc_us-gaap_IPOMember_72652af5-fe50-411b-8ac2-eea4bb116bb0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/OrganizationandDescriptionofBusiness" xlink:type="simple" xlink:href="ncno-20201031.xsd#OrganizationandDescriptionofBusiness"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/OrganizationandDescriptionofBusiness" xlink:type="extended" id="ie36146df97524b6b83edaad21c4e875c_OrganizationandDescriptionofBusiness"/>
  <link:roleRef roleURI="http://www.ncino.com/role/OrganizationandDescriptionofBusinessDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#OrganizationandDescriptionofBusinessDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/OrganizationandDescriptionofBusinessDetails" xlink:type="extended" id="i730beaf719e64a19b36c9617b419b587_OrganizationandDescriptionofBusinessDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsidiarySaleOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_7b4c7845-32ee-4640-9002-3275465809aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_7b4c7845-32ee-4640-9002-3275465809aa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_b241342e-76ed-4a37-8ab3-a40214851f0e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_b241342e-76ed-4a37-8ab3-a40214851f0e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_4380539c-6816-412b-8b02-6f69f2874e94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_4380539c-6816-412b-8b02-6f69f2874e94" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_758ae737-9eda-4a74-be65-7a2ac7deda35" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_758ae737-9eda-4a74-be65-7a2ac7deda35" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_525bb0e0-ff16-4245-bd56-8c5e6d5f3786" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_525bb0e0-ff16-4245-bd56-8c5e6d5f3786" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockPricePerShare_9cb3c5df-ff52-4311-b061-5c17b26e8f23" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_SaleOfStockPricePerShare_9cb3c5df-ff52-4311-b061-5c17b26e8f23" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_f8e8511a-4413-4d0a-aa7c-d369f290d95b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_f8e8511a-4413-4d0a-aa7c-d369f290d95b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_SaleOfStockCostsIncurredRelatedToStockIssuance_ad08cf48-18e5-4a41-a5ff-e6b38b4dba8e" xlink:href="ncno-20201031.xsd#ncno_SaleOfStockCostsIncurredRelatedToStockIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_ncno_SaleOfStockCostsIncurredRelatedToStockIssuance_ad08cf48-18e5-4a41-a5ff-e6b38b4dba8e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_SaleOfStockSharesReleasedFromLockUpAgreements_6eaf6b8b-670b-43e4-9391-8947532f3de5" xlink:href="ncno-20201031.xsd#ncno_SaleOfStockSharesReleasedFromLockUpAgreements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_ncno_SaleOfStockSharesReleasedFromLockUpAgreements_6eaf6b8b-670b-43e4-9391-8947532f3de5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_88332a82-df4b-4b71-855a-a5ddb3593ddd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_ec5bb620-120d-40f9-a797-09cc2444e0ac" xlink:to="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_88332a82-df4b-4b71-855a-a5ddb3593ddd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_c09f42c0-e035-424c-b97f-fc0edf4c7c15" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_88332a82-df4b-4b71-855a-a5ddb3593ddd" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_c09f42c0-e035-424c-b97f-fc0edf4c7c15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_c09f42c0-e035-424c-b97f-fc0edf4c7c15_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_c09f42c0-e035-424c-b97f-fc0edf4c7c15" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_c09f42c0-e035-424c-b97f-fc0edf4c7c15_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_65d7058c-2756-4212-9eb3-38efafc7cd8e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_c09f42c0-e035-424c-b97f-fc0edf4c7c15" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_65d7058c-2756-4212-9eb3-38efafc7cd8e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IPOMember_2652ef18-1f8a-496d-b14a-fdee09957344" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IPOMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_65d7058c-2756-4212-9eb3-38efafc7cd8e" xlink:to="loc_us-gaap_IPOMember_2652ef18-1f8a-496d-b14a-fdee09957344" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_SecondaryPublicOfferingMember_e5101266-2262-4e21-a62a-f44474e0f3c8" xlink:href="ncno-20201031.xsd#ncno_SecondaryPublicOfferingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_65d7058c-2756-4212-9eb3-38efafc7cd8e" xlink:to="loc_ncno_SecondaryPublicOfferingMember_e5101266-2262-4e21-a62a-f44474e0f3c8" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/SummaryofSignificantAccountingPolicies" xlink:type="simple" xlink:href="ncno-20201031.xsd#SummaryofSignificantAccountingPolicies"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SummaryofSignificantAccountingPolicies" xlink:type="extended" id="i6e03145112494c359fbbcd566371ef2d_SummaryofSignificantAccountingPolicies"/>
  <link:roleRef roleURI="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesPolicies" xlink:type="simple" xlink:href="ncno-20201031.xsd#SummaryofSignificantAccountingPoliciesPolicies"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesPolicies" xlink:type="extended" id="i36c73900a8b94f6a9a373d2b4749f362_SummaryofSignificantAccountingPoliciesPolicies"/>
  <link:roleRef roleURI="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#SummaryofSignificantAccountingPoliciesTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesTables" xlink:type="extended" id="ie588aa03c2bd4e09b91607e526c56c77_SummaryofSignificantAccountingPoliciesTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesConcentrationofCreditRiskandSignificantCustomersDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#SummaryofSignificantAccountingPoliciesConcentrationofCreditRiskandSignificantCustomersDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesConcentrationofCreditRiskandSignificantCustomersDetails" xlink:type="extended" id="i236451e39a1a4ffd96807ed3c6547850_SummaryofSignificantAccountingPoliciesConcentrationofCreditRiskandSignificantCustomersDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_30f24f17-b68b-46df-a0d5-305d064cc55d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_8243d649-2c55-43b5-bbd4-9db53382eba1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_30f24f17-b68b-46df-a0d5-305d064cc55d" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_8243d649-2c55-43b5-bbd4-9db53382eba1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_cce8ba2f-759b-4afe-a8a7-370dec5dfbe7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_30f24f17-b68b-46df-a0d5-305d064cc55d" xlink:to="loc_us-gaap_ConcentrationRiskTable_cce8ba2f-759b-4afe-a8a7-370dec5dfbe7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_0af93bd6-1614-4c2b-bd9e-6862ccf5417d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_cce8ba2f-759b-4afe-a8a7-370dec5dfbe7" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_0af93bd6-1614-4c2b-bd9e-6862ccf5417d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_0af93bd6-1614-4c2b-bd9e-6862ccf5417d_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_0af93bd6-1614-4c2b-bd9e-6862ccf5417d" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_0af93bd6-1614-4c2b-bd9e-6862ccf5417d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_a6a1fadb-1bd5-4433-9070-375041fceb04" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_0af93bd6-1614-4c2b-bd9e-6862ccf5417d" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_a6a1fadb-1bd5-4433-9070-375041fceb04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableMember_fc6c1334-0653-4511-aab5-99201caa0b42" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_a6a1fadb-1bd5-4433-9070-375041fceb04" xlink:to="loc_us-gaap_AccountsReceivableMember_fc6c1334-0653-4511-aab5-99201caa0b42" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_a8736bf4-18cb-4939-a675-ba59deb1e426" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_cce8ba2f-759b-4afe-a8a7-370dec5dfbe7" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_a8736bf4-18cb-4939-a675-ba59deb1e426" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_a8736bf4-18cb-4939-a675-ba59deb1e426_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_a8736bf4-18cb-4939-a675-ba59deb1e426" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_a8736bf4-18cb-4939-a675-ba59deb1e426_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_778cf5ce-5800-4959-9681-2c80f98e7d62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_a8736bf4-18cb-4939-a675-ba59deb1e426" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_778cf5ce-5800-4959-9681-2c80f98e7d62" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_8d7d5527-30f7-413f-a4f8-8c593cecebe7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_778cf5ce-5800-4959-9681-2c80f98e7d62" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_8d7d5527-30f7-413f-a4f8-8c593cecebe7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_fa57e7ac-96ef-489c-a105-7f4962a31067" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_cce8ba2f-759b-4afe-a8a7-370dec5dfbe7" xlink:to="loc_srt_MajorCustomersAxis_fa57e7ac-96ef-489c-a105-7f4962a31067" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_fa57e7ac-96ef-489c-a105-7f4962a31067_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_fa57e7ac-96ef-489c-a105-7f4962a31067" xlink:to="loc_srt_NameOfMajorCustomerDomain_fa57e7ac-96ef-489c-a105-7f4962a31067_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_297a81aa-693e-499f-b1fe-65258e59a2dc" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_fa57e7ac-96ef-489c-a105-7f4962a31067" xlink:to="loc_srt_NameOfMajorCustomerDomain_297a81aa-693e-499f-b1fe-65258e59a2dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_TwoCustomersMember_9a2c3ebf-1395-45f0-a58f-137feed25d05" xlink:href="ncno-20201031.xsd#ncno_TwoCustomersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_297a81aa-693e-499f-b1fe-65258e59a2dc" xlink:to="loc_ncno_TwoCustomersMember_9a2c3ebf-1395-45f0-a58f-137feed25d05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_EquityHolderMember_c3be3ee2-0255-422e-abdc-a7f9c0975a39" xlink:href="ncno-20201031.xsd#ncno_EquityHolderMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_297a81aa-693e-499f-b1fe-65258e59a2dc" xlink:to="loc_ncno_EquityHolderMember_c3be3ee2-0255-422e-abdc-a7f9c0975a39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_OneCustomerMember_d139f190-c93d-469d-b69a-092ee9004104" xlink:href="ncno-20201031.xsd#ncno_OneCustomerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_297a81aa-693e-499f-b1fe-65258e59a2dc" xlink:to="loc_ncno_OneCustomerMember_d139f190-c93d-469d-b69a-092ee9004104" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesSummaryofUncollectibleAccountsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#SummaryofSignificantAccountingPoliciesSummaryofUncollectibleAccountsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SummaryofSignificantAccountingPoliciesSummaryofUncollectibleAccountsDetails" xlink:type="extended" id="idc33a1c80eec460cbb79a8ef6f0ba42d_SummaryofSignificantAccountingPoliciesSummaryofUncollectibleAccountsDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterest" xlink:type="simple" xlink:href="ncno-20201031.xsd#VariableInterestEntityandRedeemableNonControllingInterest"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterest" xlink:type="extended" id="i88d67577433444e78d9e83e2eaf48f68_VariableInterestEntityandRedeemableNonControllingInterest"/>
  <link:roleRef roleURI="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#VariableInterestEntityandRedeemableNonControllingInterestTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestTables" xlink:type="extended" id="id7e5eaa658514781a275c97da34b1d18_VariableInterestEntityandRedeemableNonControllingInterestTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#VariableInterestEntityandRedeemableNonControllingInterestNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestNarrativeDetails" xlink:type="extended" id="i19ca9eae230b498b95d19bbf7bf85af1_VariableInterestEntityandRedeemableNonControllingInterestNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_d164f6d2-5750-47a5-b028-2f6132224fe1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToMinorityShareholders_ee309dd6-0b20-4fca-9d0e-4b001319c165" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToMinorityShareholders"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_d164f6d2-5750-47a5-b028-2f6132224fe1" xlink:to="loc_us-gaap_PaymentsToMinorityShareholders_ee309dd6-0b20-4fca-9d0e-4b001319c165" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_40f43ca7-58af-4be7-81bd-f9aa4c8cd171" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MinorityInterestOwnershipPercentageByParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_d164f6d2-5750-47a5-b028-2f6132224fe1" xlink:to="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_40f43ca7-58af-4be7-81bd-f9aa4c8cd171" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableNoncontrollingInterestEquityRedemptionValue_eea53ff8-e31c-4e16-8904-29a3a1b2bdb5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RedeemableNoncontrollingInterestEquityRedemptionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_d164f6d2-5750-47a5-b028-2f6132224fe1" xlink:to="loc_us-gaap_RedeemableNoncontrollingInterestEquityRedemptionValue_eea53ff8-e31c-4e16-8904-29a3a1b2bdb5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_7313941b-3ca7-420a-a906-322f34a06af1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_d164f6d2-5750-47a5-b028-2f6132224fe1" xlink:to="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_7313941b-3ca7-420a-a906-322f34a06af1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_b2fe8934-ad44-466a-a91e-b9499f9b04e0" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_7313941b-3ca7-420a-a906-322f34a06af1" xlink:to="loc_srt_ConsolidatedEntitiesAxis_b2fe8934-ad44-466a-a91e-b9499f9b04e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_b2fe8934-ad44-466a-a91e-b9499f9b04e0_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_b2fe8934-ad44-466a-a91e-b9499f9b04e0" xlink:to="loc_srt_ConsolidatedEntitiesDomain_b2fe8934-ad44-466a-a91e-b9499f9b04e0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_762126ee-6326-4f74-9594-a8558b189c1a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_b2fe8934-ad44-466a-a91e-b9499f9b04e0" xlink:to="loc_srt_ConsolidatedEntitiesDomain_762126ee-6326-4f74-9594-a8558b189c1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_NCinoKKMember_940458b3-4cf4-41b6-b16f-4eefaf896c45" xlink:href="ncno-20201031.xsd#ncno_NCinoKKMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_762126ee-6326-4f74-9594-a8558b189c1a" xlink:to="loc_ncno_NCinoKKMember_940458b3-4cf4-41b6-b16f-4eefaf896c45" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_b4e9b437-565e-4899-a7be-32d189d0f791" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_7313941b-3ca7-420a-a906-322f34a06af1" xlink:to="loc_srt_OwnershipAxis_b4e9b437-565e-4899-a7be-32d189d0f791" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_b4e9b437-565e-4899-a7be-32d189d0f791_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_b4e9b437-565e-4899-a7be-32d189d0f791" xlink:to="loc_srt_OwnershipDomain_b4e9b437-565e-4899-a7be-32d189d0f791_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_c77f0fc2-de27-44bb-8364-dbfd2ad56165" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_b4e9b437-565e-4899-a7be-32d189d0f791" xlink:to="loc_srt_OwnershipDomain_c77f0fc2-de27-44bb-8364-dbfd2ad56165" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_NCinoKKMember_8e930d59-cd8c-4742-a774-7d900747428f" xlink:href="ncno-20201031.xsd#ncno_NCinoKKMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_c77f0fc2-de27-44bb-8364-dbfd2ad56165" xlink:to="loc_ncno_NCinoKKMember_8e930d59-cd8c-4742-a774-7d900747428f" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestFinancialAssetsMeasuredAtFairValueDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#VariableInterestEntityandRedeemableNonControllingInterestFinancialAssetsMeasuredAtFairValueDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/VariableInterestEntityandRedeemableNonControllingInterestFinancialAssetsMeasuredAtFairValueDetails" xlink:type="extended" id="i349516befa724681b9fbd1c6fd523c98_VariableInterestEntityandRedeemableNonControllingInterestFinancialAssetsMeasuredAtFairValueDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/FairValueofFinancialInstruments" xlink:type="simple" xlink:href="ncno-20201031.xsd#FairValueofFinancialInstruments"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/FairValueofFinancialInstruments" xlink:type="extended" id="i5fa6ef259d044568a87f957f9efcfc99_FairValueofFinancialInstruments"/>
  <link:roleRef roleURI="http://www.ncino.com/role/FairValueofFinancialInstrumentsTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#FairValueofFinancialInstrumentsTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/FairValueofFinancialInstrumentsTables" xlink:type="extended" id="i850af9793111499a8e4291fbb0edd7b0_FairValueofFinancialInstrumentsTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/FairValueofFinancialInstrumentsSummaryofFinancialAssetsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#FairValueofFinancialInstrumentsSummaryofFinancialAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/FairValueofFinancialInstrumentsSummaryofFinancialAssetsDetails" xlink:type="extended" id="i5cbe52b9aa054e7185adc02f7d592933_FairValueofFinancialInstrumentsSummaryofFinancialAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_f71b1658-048a-452e-81c2-ec341897b611" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosureAbstract_5c1f0d94-61ac-4c18-9d38-2a53d3e3393d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_f71b1658-048a-452e-81c2-ec341897b611" xlink:to="loc_us-gaap_AssetsFairValueDisclosureAbstract_5c1f0d94-61ac-4c18-9d38-2a53d3e3393d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_cfb0023a-cb26-407a-990d-bcffd052625d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_5c1f0d94-61ac-4c18-9d38-2a53d3e3393d" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_cfb0023a-cb26-407a-990d-bcffd052625d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_b412b3e2-9d1a-483a-aa63-ea0abc26402b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_5c1f0d94-61ac-4c18-9d38-2a53d3e3393d" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_b412b3e2-9d1a-483a-aa63-ea0abc26402b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_70982fce-2d5e-472f-9970-63e55890a757" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_f71b1658-048a-452e-81c2-ec341897b611" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_70982fce-2d5e-472f-9970-63e55890a757" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations_231e304b-9add-4522-9e44-9bfa0254dc60" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_70982fce-2d5e-472f-9970-63e55890a757" xlink:to="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations_231e304b-9add-4522-9e44-9bfa0254dc60" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_241a8057-283c-400e-903e-04219d19e4ce" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_70982fce-2d5e-472f-9970-63e55890a757" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_241a8057-283c-400e-903e-04219d19e4ce" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e6aa4604-c4d6-45e1-bb6d-1e488a7614fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_f71b1658-048a-452e-81c2-ec341897b611" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e6aa4604-c4d6-45e1-bb6d-1e488a7614fd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ef46269a-d396-44c5-adb6-886b4835f5c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e6aa4604-c4d6-45e1-bb6d-1e488a7614fd" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ef46269a-d396-44c5-adb6-886b4835f5c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_ef46269a-d396-44c5-adb6-886b4835f5c3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ef46269a-d396-44c5-adb6-886b4835f5c3" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_ef46269a-d396-44c5-adb6-886b4835f5c3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_adc65751-1491-421e-b78c-a23012df4a20" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ef46269a-d396-44c5-adb6-886b4835f5c3" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_adc65751-1491-421e-b78c-a23012df4a20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_3d19fbfc-c364-4d4d-950a-ca784bc66ce5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_adc65751-1491-421e-b78c-a23012df4a20" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_3d19fbfc-c364-4d4d-950a-ca784bc66ce5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_7d27a0c8-519b-4052-a11d-6bba74f2d392" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_adc65751-1491-421e-b78c-a23012df4a20" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_7d27a0c8-519b-4052-a11d-6bba74f2d392" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_b6bce93c-475c-49a4-bc8c-b29bddf286fe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_adc65751-1491-421e-b78c-a23012df4a20" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_b6bce93c-475c-49a4-bc8c-b29bddf286fe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e6aa4604-c4d6-45e1-bb6d-1e488a7614fd" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d1090e1-d31f-4000-a8c0-f28b87ca02e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_80d7f4b4-106d-4ba5-ab75-2f84fdb28cd0" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d1090e1-d31f-4000-a8c0-f28b87ca02e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_37534f42-0a77-4e59-a5dd-b52ae161f6f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d1090e1-d31f-4000-a8c0-f28b87ca02e2" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_37534f42-0a77-4e59-a5dd-b52ae161f6f5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAxis_7a499589-72af-4c77-b639-2ce07e86cb62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e6aa4604-c4d6-45e1-bb6d-1e488a7614fd" xlink:to="loc_us-gaap_CashAndCashEquivalentsAxis_7a499589-72af-4c77-b639-2ce07e86cb62" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_7a499589-72af-4c77-b639-2ce07e86cb62_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_7a499589-72af-4c77-b639-2ce07e86cb62" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_7a499589-72af-4c77-b639-2ce07e86cb62_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_b46d4b00-53f5-4d99-81c0-bbecf1910e83" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_7a499589-72af-4c77-b639-2ce07e86cb62" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_b46d4b00-53f5-4d99-81c0-bbecf1910e83" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_f133642a-4b20-4e94-a76d-e62e658ac5aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_b46d4b00-53f5-4d99-81c0-bbecf1910e83" xlink:to="loc_us-gaap_MoneyMarketFundsMember_f133642a-4b20-4e94-a76d-e62e658ac5aa" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/FairValueofFinancialInstrumentsReconciliationforContingentConsiderationObligationsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#FairValueofFinancialInstrumentsReconciliationforContingentConsiderationObligationsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/FairValueofFinancialInstrumentsReconciliationforContingentConsiderationObligationsDetails" xlink:type="extended" id="ibf9887b900c84bb09daee8a166215d81_FairValueofFinancialInstrumentsReconciliationforContingentConsiderationObligationsDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/Revenues" xlink:type="simple" xlink:href="ncno-20201031.xsd#Revenues"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/Revenues" xlink:type="extended" id="ie01bed48dd5f4704898b48aeb4504e7d_Revenues"/>
  <link:roleRef roleURI="http://www.ncino.com/role/RevenuesTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#RevenuesTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RevenuesTables" xlink:type="extended" id="i6851b180f9c24f86998c51ef9934096c_RevenuesTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/RevenuesRevenueByGeographicRegionDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#RevenuesRevenueByGeographicRegionDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RevenuesRevenueByGeographicRegionDetails" xlink:type="extended" id="id7b3778dd2bf436db5574dec6bdaac4b_RevenuesRevenueByGeographicRegionDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_35d20e8f-e6a9-4963-8b29-1e422e5e03ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_e4dfc87f-b0ce-42cf-8d79-83997c1e41f8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_35d20e8f-e6a9-4963-8b29-1e422e5e03ca" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_e4dfc87f-b0ce-42cf-8d79-83997c1e41f8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_b7b05d5e-ce18-426e-a39e-455fb6e122c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_35d20e8f-e6a9-4963-8b29-1e422e5e03ca" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_b7b05d5e-ce18-426e-a39e-455fb6e122c6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_b7b05d5e-ce18-426e-a39e-455fb6e122c6" xlink:to="loc_srt_StatementGeographicalAxis_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa" xlink:to="loc_srt_SegmentGeographicalDomain_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_59dc1073-bd53-4f6b-8468-d72390c2a652" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_5b3d22b9-0b5d-42e5-9571-9b7bc7604cfa" xlink:to="loc_srt_SegmentGeographicalDomain_59dc1073-bd53-4f6b-8468-d72390c2a652" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_3e8f7d56-f1cb-45eb-82d6-a5674be3fe47" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_59dc1073-bd53-4f6b-8468-d72390c2a652" xlink:to="loc_country_US_3e8f7d56-f1cb-45eb-82d6-a5674be3fe47" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_04d800ec-d510-4cca-b341-902ac84c3683" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_59dc1073-bd53-4f6b-8468-d72390c2a652" xlink:to="loc_us-gaap_NonUsMember_04d800ec-d510-4cca-b341-902ac84c3683" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/RevenuesAccountsReceivableLessAllowanceforDoubtfulAccountsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#RevenuesAccountsReceivableLessAllowanceforDoubtfulAccountsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RevenuesAccountsReceivableLessAllowanceforDoubtfulAccountsDetails" xlink:type="extended" id="i242485d42d6b4822bcd217c46fe87e6b_RevenuesAccountsReceivableLessAllowanceforDoubtfulAccountsDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/RevenuesNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#RevenuesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RevenuesNarrativeDetails" xlink:type="extended" id="id70655eeb5954a7da48c00bea5a4d055_RevenuesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties_d3b996ed-f831-48cb-9655-5fc6de60e576" xlink:href="ncno-20201031.xsd#ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties_d3b996ed-f831-48cb-9655-5fc6de60e576" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_1c863a06-677b-4338-9aea-07ba638fb07e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_1c863a06-677b-4338-9aea-07ba638fb07e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_7323afa7-91bd-4aac-9237-3eb0b5e9a186" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_7323afa7-91bd-4aac-9237-3eb0b5e9a186" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_6aa00d34-3238-47ec-abc4-53e14bcdb5e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_6aa00d34-3238-47ec-abc4-53e14bcdb5e2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:to="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_de86509f-317a-4392-ad59-f7dfcb5e58d4_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:to="loc_srt_RangeMember_de86509f-317a-4392-ad59-f7dfcb5e58d4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:to="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_945b406d-48f7-4f48-8f06-ae6859c7a53f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:to="loc_srt_MinimumMember_945b406d-48f7-4f48-8f06-ae6859c7a53f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_853968b6-30d6-4853-8a22-cffa0d4f0d01" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:to="loc_srt_MaximumMember_853968b6-30d6-4853-8a22-cffa0d4f0d01" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_5a206d92-47cb-4410-af8c-0dfe07ada6d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_5a206d92-47cb-4410-af8c-0dfe07ada6d7" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/RevenuesNarrativeDetails_1" xlink:type="simple" xlink:href="ncno-20201031.xsd#RevenuesNarrativeDetails_1"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RevenuesNarrativeDetails_1" xlink:type="extended" id="i09d461e651e145f690d2a090e383b07f_RevenuesNarrativeDetails_1">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties_d3b996ed-f831-48cb-9655-5fc6de60e576" xlink:href="ncno-20201031.xsd#ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_ncno_ContractWithCustomerLiabilityRevenueRecognizedIncludingRelatedParties_d3b996ed-f831-48cb-9655-5fc6de60e576" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_1c863a06-677b-4338-9aea-07ba638fb07e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_1c863a06-677b-4338-9aea-07ba638fb07e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_7323afa7-91bd-4aac-9237-3eb0b5e9a186" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_7323afa7-91bd-4aac-9237-3eb0b5e9a186" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_6aa00d34-3238-47ec-abc4-53e14bcdb5e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_6aa00d34-3238-47ec-abc4-53e14bcdb5e2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d2984e96-22a3-430b-83d8-7a411924b28f" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_634986b7-963f-4733-b11b-23f5e598cb19" xlink:to="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_de86509f-317a-4392-ad59-f7dfcb5e58d4_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:to="loc_srt_RangeMember_de86509f-317a-4392-ad59-f7dfcb5e58d4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_de86509f-317a-4392-ad59-f7dfcb5e58d4" xlink:to="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_945b406d-48f7-4f48-8f06-ae6859c7a53f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:to="loc_srt_MinimumMember_945b406d-48f7-4f48-8f06-ae6859c7a53f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_853968b6-30d6-4853-8a22-cffa0d4f0d01" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cce8fd1d-4c0b-4643-9be0-f96065115d17" xlink:to="loc_srt_MaximumMember_853968b6-30d6-4853-8a22-cffa0d4f0d01" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/BusinessCombinations" xlink:type="simple" xlink:href="ncno-20201031.xsd#BusinessCombinations"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BusinessCombinations" xlink:type="extended" id="i5d2c9ae1b1bd4fab9f9f83c872102d6b_BusinessCombinations"/>
  <link:roleRef roleURI="http://www.ncino.com/role/BusinessCombinationsTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#BusinessCombinationsTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BusinessCombinationsTables" xlink:type="extended" id="i0c306c4b10ce432ea700f728b88510d1_BusinessCombinationsTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/BusinessCombinationsAcquisitionofVisibleEquityLLCConsiderationTransferredDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BusinessCombinationsAcquisitionofVisibleEquityLLCConsiderationTransferredDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BusinessCombinationsAcquisitionofVisibleEquityLLCConsiderationTransferredDetails" xlink:type="extended" id="i6ee10b558fa84401910c8826e5af4775_BusinessCombinationsAcquisitionofVisibleEquityLLCConsiderationTransferredDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_a9be63f1-c7ad-4462-8cd7-22be33bd41f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_a9be63f1-c7ad-4462-8cd7-22be33bd41f1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_b7533ec9-d594-4add-84c2-531659ec6cf2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_b7533ec9-d594-4add-84c2-531659ec6cf2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_090a0701-bd56-4a02-a2ec-c1349eaadc95" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_090a0701-bd56-4a02-a2ec-c1349eaadc95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_ae571272-c2c8-495b-9a9f-d347266ef9e5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_ae571272-c2c8-495b-9a9f-d347266ef9e5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_dd3dbcff-7e79-41ea-ac2f-178a801170cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_a37e51f2-a550-4803-b930-f27992f391b4" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_dd3dbcff-7e79-41ea-ac2f-178a801170cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_f36d231d-11c5-4412-a7b1-127f6ee2008e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_dd3dbcff-7e79-41ea-ac2f-178a801170cd" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_f36d231d-11c5-4412-a7b1-127f6ee2008e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f36d231d-11c5-4412-a7b1-127f6ee2008e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_f36d231d-11c5-4412-a7b1-127f6ee2008e" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f36d231d-11c5-4412-a7b1-127f6ee2008e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_885458d6-ea20-411f-aabd-e972016b7b81" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_f36d231d-11c5-4412-a7b1-127f6ee2008e" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_885458d6-ea20-411f-aabd-e972016b7b81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VisibleEquityLLCMember_be1b1fe0-2bcf-409c-ad2f-987a927ebf52" xlink:href="ncno-20201031.xsd#ncno_VisibleEquityLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_885458d6-ea20-411f-aabd-e972016b7b81" xlink:to="loc_ncno_VisibleEquityLLCMember_be1b1fe0-2bcf-409c-ad2f-987a927ebf52" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_dd3dbcff-7e79-41ea-ac2f-178a801170cd" xlink:to="loc_us-gaap_StatementClassOfStockAxis_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01" xlink:to="loc_us-gaap_ClassOfStockDomain_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_0fef7e3b-6cb7-4f36-8f89-61987dd3ecf3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f5571e36-f1a8-4594-ab0a-b2bd3e5abd01" xlink:to="loc_us-gaap_ClassOfStockDomain_0fef7e3b-6cb7-4f36-8f89-61987dd3ecf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VotingCommonStockMember_8f0474a0-4530-4416-b91c-d9ebe04ae5b1" xlink:href="ncno-20201031.xsd#ncno_VotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_0fef7e3b-6cb7-4f36-8f89-61987dd3ecf3" xlink:to="loc_ncno_VotingCommonStockMember_8f0474a0-4530-4416-b91c-d9ebe04ae5b1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/BusinessCombinationsAcquisitionofFinsuitePtyLtdConsiderationTransferredDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BusinessCombinationsAcquisitionofFinsuitePtyLtdConsiderationTransferredDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BusinessCombinationsAcquisitionofFinsuitePtyLtdConsiderationTransferredDetails" xlink:type="extended" id="i78ee7873e4e34340a6f71f4768b448c9_BusinessCombinationsAcquisitionofFinsuitePtyLtdConsiderationTransferredDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_ca41c88e-386f-427f-898e-13374847973a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_ca41c88e-386f-427f-898e-13374847973a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferredLiabilitiesIncurred_e6820970-3c90-41e2-b0d7-0e9bf230a363" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferredLiabilitiesIncurred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferredLiabilitiesIncurred_e6820970-3c90-41e2-b0d7-0e9bf230a363" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_5fb8e1fa-b670-48f8-9a01-0e879c618259" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_5fb8e1fa-b670-48f8-9a01-0e879c618259" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_5bbdc694-d983-43ca-8880-b8fd1b7cbcbf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_5bbdc694-d983-43ca-8880-b8fd1b7cbcbf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_BusinessCombinationConsiderationTransferredCashPaid_4cedc062-7640-4eb7-b917-31deeb040de7" xlink:href="ncno-20201031.xsd#ncno_BusinessCombinationConsiderationTransferredCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_ncno_BusinessCombinationConsiderationTransferredCashPaid_4cedc062-7640-4eb7-b917-31deeb040de7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_d6e145ab-249f-4434-b81d-c3e92e9842dc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_d6e145ab-249f-4434-b81d-c3e92e9842dc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_327b8531-1cf7-459f-9eb6-2600d2001a90" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_eddf180b-c5e1-4cee-ae60-aac7567f7222" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_327b8531-1cf7-459f-9eb6-2600d2001a90" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_bf25faf9-d3fb-4e05-9e50-7a694c5d4082" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_327b8531-1cf7-459f-9eb6-2600d2001a90" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_bf25faf9-d3fb-4e05-9e50-7a694c5d4082" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_bf25faf9-d3fb-4e05-9e50-7a694c5d4082_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_bf25faf9-d3fb-4e05-9e50-7a694c5d4082" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_bf25faf9-d3fb-4e05-9e50-7a694c5d4082_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_185e1c34-69bb-4520-8468-43a5e2415d04" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_bf25faf9-d3fb-4e05-9e50-7a694c5d4082" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_185e1c34-69bb-4520-8468-43a5e2415d04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FinSuitePtyLtdMember_b94f71bf-e42b-4761-b039-1c926a32069c" xlink:href="ncno-20201031.xsd#ncno_FinSuitePtyLtdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_185e1c34-69bb-4520-8468-43a5e2415d04" xlink:to="loc_ncno_FinSuitePtyLtdMember_b94f71bf-e42b-4761-b039-1c926a32069c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_327b8531-1cf7-459f-9eb6-2600d2001a90" xlink:to="loc_us-gaap_StatementClassOfStockAxis_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2" xlink:to="loc_us-gaap_ClassOfStockDomain_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_367c8951-70b2-4049-a3aa-f47a22a24d11" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5db62773-ff8a-4a6f-a78c-a9d328cd7fa2" xlink:to="loc_us-gaap_ClassOfStockDomain_367c8951-70b2-4049-a3aa-f47a22a24d11" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_VotingCommonStockMember_53e146a8-0df2-4de4-b6ff-19f21d56cf4b" xlink:href="ncno-20201031.xsd#ncno_VotingCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_367c8951-70b2-4049-a3aa-f47a22a24d11" xlink:to="loc_ncno_VotingCommonStockMember_53e146a8-0df2-4de4-b6ff-19f21d56cf4b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/BusinessCombinationsNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BusinessCombinationsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BusinessCombinationsNarrativeDetails" xlink:type="extended" id="i024e497bf5674c46b04778cdfe141990_BusinessCombinationsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionContingentConsiderationLineItems_3983735b-600c-43dd-b4a1-a8884d16c7b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionContingentConsiderationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_BusinessCombinationConsiderationTransferredCashPaid_b5b42a73-2e48-47b6-9868-1c0278ce68d1" xlink:href="ncno-20201031.xsd#ncno_BusinessCombinationConsiderationTransferredCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionContingentConsiderationLineItems_3983735b-600c-43dd-b4a1-a8884d16c7b3" xlink:to="loc_ncno_BusinessCombinationConsiderationTransferredCashPaid_b5b42a73-2e48-47b6-9868-1c0278ce68d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_f59287e0-9624-4c12-98d1-df9fb8283416" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionContingentConsiderationLineItems_3983735b-600c-43dd-b4a1-a8884d16c7b3" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_f59287e0-9624-4c12-98d1-df9fb8283416" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionContingentConsiderationTable_cead5b47-ae2b-4a6f-b37c-360b551664cf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionContingentConsiderationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionContingentConsiderationLineItems_3983735b-600c-43dd-b4a1-a8884d16c7b3" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionContingentConsiderationTable_cead5b47-ae2b-4a6f-b37c-360b551664cf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationByTypeAxis_94429130-9efc-465a-afca-60d63c9bc911" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionContingentConsiderationTable_cead5b47-ae2b-4a6f-b37c-360b551664cf" xlink:to="loc_us-gaap_ContingentConsiderationByTypeAxis_94429130-9efc-465a-afca-60d63c9bc911" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_94429130-9efc-465a-afca-60d63c9bc911_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_94429130-9efc-465a-afca-60d63c9bc911" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_94429130-9efc-465a-afca-60d63c9bc911_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_a4b5ff83-6c25-4aba-8e53-d3339be8c18e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_94429130-9efc-465a-afca-60d63c9bc911" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_a4b5ff83-6c25-4aba-8e53-d3339be8c18e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FirstInitialTrancheEarnOutMember_71222458-3fd2-48f2-9b3c-a6ed5be4b8c4" xlink:href="ncno-20201031.xsd#ncno_FirstInitialTrancheEarnOutMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_a4b5ff83-6c25-4aba-8e53-d3339be8c18e" xlink:to="loc_ncno_FirstInitialTrancheEarnOutMember_71222458-3fd2-48f2-9b3c-a6ed5be4b8c4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_SecondInitialTrancheEarnOutMember_ca8131b6-b017-4663-8a8e-458b75bab76c" xlink:href="ncno-20201031.xsd#ncno_SecondInitialTrancheEarnOutMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_a4b5ff83-6c25-4aba-8e53-d3339be8c18e" xlink:to="loc_ncno_SecondInitialTrancheEarnOutMember_ca8131b6-b017-4663-8a8e-458b75bab76c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FirstAndSecondTrancheEarnOutMember_27112efc-7975-4eba-a61f-6004b5100de5" xlink:href="ncno-20201031.xsd#ncno_FirstAndSecondTrancheEarnOutMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_a4b5ff83-6c25-4aba-8e53-d3339be8c18e" xlink:to="loc_ncno_FirstAndSecondTrancheEarnOutMember_27112efc-7975-4eba-a61f-6004b5100de5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_b767087c-25b4-4881-be58-e6126051917e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionContingentConsiderationTable_cead5b47-ae2b-4a6f-b37c-360b551664cf" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_b767087c-25b4-4881-be58-e6126051917e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_b767087c-25b4-4881-be58-e6126051917e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b767087c-25b4-4881-be58-e6126051917e" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_b767087c-25b4-4881-be58-e6126051917e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_b1271514-7662-4767-a512-511bd88c3d10" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b767087c-25b4-4881-be58-e6126051917e" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_b1271514-7662-4767-a512-511bd88c3d10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_6d0873c5-5cf7-4487-aa4e-7654970fc90d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_b1271514-7662-4767-a512-511bd88c3d10" xlink:to="loc_us-gaap_SubsequentEventMember_6d0873c5-5cf7-4487-aa4e-7654970fc90d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwill" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwill"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwill" xlink:type="extended" id="i7c46bdc1d2944ddf8fc66c98e58dff09_IntangibleAssetsandGoodwill"/>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillTables" xlink:type="extended" id="i4f70fff28f3b45f39f2beac119fc3ebf_IntangibleAssetsandGoodwillTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillScheduleofIntangibleAssetsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillScheduleofIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillScheduleofIntangibleAssetsDetails" xlink:type="extended" id="ie1d81b84837646d78e44bfdfa31dae3b_IntangibleAssetsandGoodwillScheduleofIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_59348ac9-bb0c-4acb-9477-f256fe67e0eb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_558af612-2275-4d4a-a10c-98893bae0597" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_59348ac9-bb0c-4acb-9477-f256fe67e0eb" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_558af612-2275-4d4a-a10c-98893bae0597" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_51f45308-90b5-4e9f-aaf7-3999499c4a7c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_59348ac9-bb0c-4acb-9477-f256fe67e0eb" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_51f45308-90b5-4e9f-aaf7-3999499c4a7c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b1365c70-6df0-4df7-bb76-eb63191abac1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_59348ac9-bb0c-4acb-9477-f256fe67e0eb" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b1365c70-6df0-4df7-bb76-eb63191abac1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_bb8f134c-0079-483f-9a3d-f1dfa4e9e06c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_59348ac9-bb0c-4acb-9477-f256fe67e0eb" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_bb8f134c-0079-483f-9a3d-f1dfa4e9e06c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_bb8f134c-0079-483f-9a3d-f1dfa4e9e06c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_84757b54-8234-4de5-9167-f0d734669b7f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1d3cd9f0-32cb-4a6d-af7f-a4cf35ae8e77" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_84757b54-8234-4de5-9167-f0d734669b7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DevelopedTechnologyRightsMember_f5871806-49d1-4eb9-94a9-90c40b4c188d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DevelopedTechnologyRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_84757b54-8234-4de5-9167-f0d734669b7f" xlink:to="loc_us-gaap_DevelopedTechnologyRightsMember_f5871806-49d1-4eb9-94a9-90c40b4c188d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_0e839d8a-70f0-4fe4-9989-90fa70f2d792" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_84757b54-8234-4de5-9167-f0d734669b7f" xlink:to="loc_us-gaap_CustomerRelationshipsMember_0e839d8a-70f0-4fe4-9989-90fa70f2d792" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksMember_63759149-f1de-433d-ba9d-c051cc4cae5f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TrademarksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_84757b54-8234-4de5-9167-f0d734669b7f" xlink:to="loc_us-gaap_TrademarksMember_63759149-f1de-433d-ba9d-c051cc4cae5f" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillAmortizationExpenseDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillAmortizationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillAmortizationExpenseDetails" xlink:type="extended" id="i6431c488924c49b1b4d6d9055956ab8e_IntangibleAssetsandGoodwillAmortizationExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_3e0d2f01-6837-435f-9b90-8876f02d9282" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_0a268f2a-3fee-48f2-a319-1e52b82f7f5c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_3e0d2f01-6837-435f-9b90-8876f02d9282" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_0a268f2a-3fee-48f2-a319-1e52b82f7f5c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d80c2af6-c777-422e-afb7-2b3cb719e90e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_3e0d2f01-6837-435f-9b90-8876f02d9282" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d80c2af6-c777-422e-afb7-2b3cb719e90e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_94b2e385-b470-4788-991b-9e4d63a5d87c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d80c2af6-c777-422e-afb7-2b3cb719e90e" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_94b2e385-b470-4788-991b-9e4d63a5d87c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_94b2e385-b470-4788-991b-9e4d63a5d87c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_94b2e385-b470-4788-991b-9e4d63a5d87c" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_94b2e385-b470-4788-991b-9e4d63a5d87c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_066a7b99-f1d4-4501-9dc7-67fe6a0e2143" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_94b2e385-b470-4788-991b-9e4d63a5d87c" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_066a7b99-f1d4-4501-9dc7-67fe6a0e2143" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_b5f24f58-1126-4395-8638-86fb01aefc43" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_066a7b99-f1d4-4501-9dc7-67fe6a0e2143" xlink:to="loc_us-gaap_CostOfSalesMember_b5f24f58-1126-4395-8638-86fb01aefc43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_3785ea54-a084-4422-83c3-1515d8b642aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_066a7b99-f1d4-4501-9dc7-67fe6a0e2143" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_3785ea54-a084-4422-83c3-1515d8b642aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_bcb8c511-ca3d-4e50-8e58-4e09e1c26c71" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_066a7b99-f1d4-4501-9dc7-67fe6a0e2143" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_bcb8c511-ca3d-4e50-8e58-4e09e1c26c71" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillExpectedFutureAmortizationExpenseDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillExpectedFutureAmortizationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillExpectedFutureAmortizationExpenseDetails" xlink:type="extended" id="i1ec49dd2316e401292f41c095898060e_IntangibleAssetsandGoodwillExpectedFutureAmortizationExpenseDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillNarrativeDetails" xlink:type="extended" id="ic2eda2d62973466ca2235b39cb71302c_IntangibleAssetsandGoodwillNarrativeDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/IntangibleAssetsandGoodwillScheduleofGoodwillDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#IntangibleAssetsandGoodwillScheduleofGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/IntangibleAssetsandGoodwillScheduleofGoodwillDetails" xlink:type="extended" id="id62f3f29b19a49c8841430b4a4183b5c_IntangibleAssetsandGoodwillScheduleofGoodwillDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/ResellerAgreement" xlink:type="simple" xlink:href="ncno-20201031.xsd#ResellerAgreement"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ResellerAgreement" xlink:type="extended" id="i1cf20767499b455ba845711a14822c55_ResellerAgreement"/>
  <link:roleRef roleURI="http://www.ncino.com/role/ResellerAgreementNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#ResellerAgreementNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/ResellerAgreementNarrativeDetails" xlink:type="extended" id="i9c3051538d8c4e66bc3adcd632e2928e_ResellerAgreementNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_d0da6462-47a9-4f3d-a819-5f71f32b5334" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyCosts_9c26ae86-0be9-431b-b8e4-0c84f4dd7050" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d0da6462-47a9-4f3d-a819-5f71f32b5334" xlink:to="loc_us-gaap_RelatedPartyCosts_9c26ae86-0be9-431b-b8e4-0c84f4dd7050" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_7ad7ed82-73c8-4678-9b6a-ab36f3242be6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d0da6462-47a9-4f3d-a819-5f71f32b5334" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_7ad7ed82-73c8-4678-9b6a-ab36f3242be6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2f230bb7-ef19-4b95-b7fd-4e8095f9d230" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_7ad7ed82-73c8-4678-9b6a-ab36f3242be6" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2f230bb7-ef19-4b95-b7fd-4e8095f9d230" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_2f230bb7-ef19-4b95-b7fd-4e8095f9d230_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2f230bb7-ef19-4b95-b7fd-4e8095f9d230" xlink:to="loc_us-gaap_RelatedPartyDomain_2f230bb7-ef19-4b95-b7fd-4e8095f9d230_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_ae0b176e-277b-4097-9925-82e13b5ba274" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2f230bb7-ef19-4b95-b7fd-4e8095f9d230" xlink:to="loc_us-gaap_RelatedPartyDomain_ae0b176e-277b-4097-9925-82e13b5ba274" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_e937175d-d6e4-47f2-8542-42b599614d7d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_ae0b176e-277b-4097-9925-82e13b5ba274" xlink:to="loc_srt_AffiliatedEntityMember_e937175d-d6e4-47f2-8542-42b599614d7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_7d900f5d-b5aa-4c26-b908-7388d71febdd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_7ad7ed82-73c8-4678-9b6a-ab36f3242be6" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_7d900f5d-b5aa-4c26-b908-7388d71febdd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_7d900f5d-b5aa-4c26-b908-7388d71febdd_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_7d900f5d-b5aa-4c26-b908-7388d71febdd" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_7d900f5d-b5aa-4c26-b908-7388d71febdd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_a093fd00-7674-44ee-9912-e9de6da8c1a0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_7d900f5d-b5aa-4c26-b908-7388d71febdd" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_a093fd00-7674-44ee-9912-e9de6da8c1a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ResellerAgreementMember_d1488b10-b92f-4d6b-bc4e-4d87a581981a" xlink:href="ncno-20201031.xsd#ncno_ResellerAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_a093fd00-7674-44ee-9912-e9de6da8c1a0" xlink:to="loc_ncno_ResellerAgreementMember_d1488b10-b92f-4d6b-bc4e-4d87a581981a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/StockholdersEquity" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockholdersEquity" xlink:type="extended" id="iedb342aa1d924432ad695bacd0727fd8_StockholdersEquity"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockholdersEquityTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockholdersEquityTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockholdersEquityTables" xlink:type="extended" id="i48a486f40d314926871f3f1a460afeb4_StockholdersEquityTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockholdersEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockholdersEquityNarrativeDetails" xlink:type="extended" id="if55bd86e381740bf86770aef78f6e07b_StockholdersEquityNarrativeDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockholdersEquityCommonStockFutureIssuanceDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockholdersEquityCommonStockFutureIssuanceDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockholdersEquityCommonStockFutureIssuanceDetails" xlink:type="extended" id="i18b8a9cf051b4cb084e9cbe474000d96_StockholdersEquityCommonStockFutureIssuanceDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_5f5114e2-34c0-499a-a080-09b5a5726e78" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_5f5114e2-34c0-499a-a080-09b5a5726e78" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_8732c4a3-0c5d-4365-943e-31260e8b1d56" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_8732c4a3-0c5d-4365-943e-31260e8b1d56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsIssuedAndOutstandingAndNotIssuedAndOutstanding_e16f582c-ae65-4afb-8311-fa2649facd6f" xlink:href="ncno-20201031.xsd#ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsIssuedAndOutstandingAndNotIssuedAndOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:to="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsIssuedAndOutstandingAndNotIssuedAndOutstanding_e16f582c-ae65-4afb-8311-fa2649facd6f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_CommonStockPossibleIssuanceOfCapitalShares_67de7b3f-38d1-41dc-b974-e7a26b2c1a13" xlink:href="ncno-20201031.xsd#ncno_CommonStockPossibleIssuanceOfCapitalShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:to="loc_ncno_CommonStockPossibleIssuanceOfCapitalShares_67de7b3f-38d1-41dc-b974-e7a26b2c1a13" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_de6fbef7-60ae-4349-8f38-cb289aa88bac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_54e54062-9b03-41be-9dfc-88f9a1508107" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a31fb83c-cf2e-4bd1-9fff-188f7a6a75df" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_54e54062-9b03-41be-9dfc-88f9a1508107" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_54e54062-9b03-41be-9dfc-88f9a1508107" xlink:to="loc_us-gaap_StatementClassOfStockAxis_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87" xlink:to="loc_us-gaap_ClassOfStockDomain_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_d230a97a-9cec-4e0e-86ef-2fddf2874d89" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fb6fa22b-33b6-4e5c-a1ac-4e07c91ecf87" xlink:to="loc_us-gaap_ClassOfStockDomain_d230a97a-9cec-4e0e-86ef-2fddf2874d89" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_21288b39-d612-4429-93fc-84d8cfe509c1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_d230a97a-9cec-4e0e-86ef-2fddf2874d89" xlink:to="loc_us-gaap_CommonStockMember_21288b39-d612-4429-93fc-84d8cfe509c1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_cdb576c2-6f37-45ae-a317-43899de4d9e7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_54e54062-9b03-41be-9dfc-88f9a1508107" xlink:to="loc_us-gaap_AwardTypeAxis_cdb576c2-6f37-45ae-a317-43899de4d9e7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_cdb576c2-6f37-45ae-a317-43899de4d9e7_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_cdb576c2-6f37-45ae-a317-43899de4d9e7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_cdb576c2-6f37-45ae-a317-43899de4d9e7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6ed0d7ab-37b5-46df-9e30-7be1e86cd1c8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_cdb576c2-6f37-45ae-a317-43899de4d9e7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6ed0d7ab-37b5-46df-9e30-7be1e86cd1c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_382323e0-d953-470c-8dc9-4218da422e51" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6ed0d7ab-37b5-46df-9e30-7be1e86cd1c8" xlink:to="loc_us-gaap_EmployeeStockOptionMember_382323e0-d953-470c-8dc9-4218da422e51" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_68101a2b-fe88-4be9-b2fa-b97f04bd7380" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6ed0d7ab-37b5-46df-9e30-7be1e86cd1c8" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_68101a2b-fe88-4be9-b2fa-b97f04bd7380" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/StockBasedCompensation" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockBasedCompensation"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockBasedCompensation" xlink:type="extended" id="iec63ddfbbc2440b0bfb2142698c32c09_StockBasedCompensation"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockBasedCompensationTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockBasedCompensationTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockBasedCompensationTables" xlink:type="extended" id="i573d7ee57a8349f49f0b2be2a8d08f69_StockBasedCompensationTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockBasedCompensationNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockBasedCompensationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockBasedCompensationNarrativeDetails" xlink:type="extended" id="i09943dcce88f4af98bc37809a51e73cf_StockBasedCompensationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_20374d87-eae7-411b-9859-2db7373bd33a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_20374d87-eae7-411b-9859-2db7373bd33a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentIncreaseInAggregateShares_14b84bed-1cec-42f5-8110-5b0a3177bf9d" xlink:href="ncno-20201031.xsd#ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentIncreaseInAggregateShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentIncreaseInAggregateShares_14b84bed-1cec-42f5-8110-5b0a3177bf9d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_63c5464e-b3a7-4a2f-9c88-1e44392b8023" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_63c5464e-b3a7-4a2f-9c88-1e44392b8023" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_46de6020-c195-486a-973a-a17f16fc741a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_46de6020-c195-486a-973a-a17f16fc741a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_082a9f4c-ca0a-4dcc-84c8-2f49f5e85b9e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_082a9f4c-ca0a-4dcc-84c8-2f49f5e85b9e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_6f6c8a36-21b4-41e5-b691-baafef819861" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_6f6c8a36-21b4-41e5-b691-baafef819861" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_42f66822-6968-4deb-a993-83f8891c6567" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_42f66822-6968-4deb-a993-83f8891c6567" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_45fc6e19-6054-4c99-b5e0-186586ac7dc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_45fc6e19-6054-4c99-b5e0-186586ac7dc6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_8d208452-b11d-4781-98c0-2e21fa168e3c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_8d208452-b11d-4781-98c0-2e21fa168e3c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_3c56f50e-3241-40fe-94b8-c2e59042e3f7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_db1d0d7d-dea1-4596-a459-7f485f342a3f" xlink:to="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_3c56f50e-3241-40fe-94b8-c2e59042e3f7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_e2e143cf-6329-475c-93b8-73145ce908b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_3c56f50e-3241-40fe-94b8-c2e59042e3f7" xlink:to="loc_us-gaap_AwardTypeAxis_e2e143cf-6329-475c-93b8-73145ce908b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e2e143cf-6329-475c-93b8-73145ce908b3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_e2e143cf-6329-475c-93b8-73145ce908b3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e2e143cf-6329-475c-93b8-73145ce908b3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_e2e143cf-6329-475c-93b8-73145ce908b3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockCompensationPlanMember_59581c34-3c4a-4c4f-9c32-73d378668a60" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockCompensationPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:to="loc_us-gaap_StockCompensationPlanMember_59581c34-3c4a-4c4f-9c32-73d378668a60" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_67308a16-711e-4196-9deb-d21793468e94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:to="loc_us-gaap_EmployeeStockOptionMember_67308a16-711e-4196-9deb-d21793468e94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_0782da7b-199f-4ee4-bb3d-6ef18b160100" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_0782da7b-199f-4ee4-bb3d-6ef18b160100" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_f3e00b91-7902-4004-8285-826842472240" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4e5c07b5-9c2c-438b-8ac2-679fffed6e17" xlink:to="loc_us-gaap_EmployeeStockMember_f3e00b91-7902-4004-8285-826842472240" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_9aa52518-2eeb-449f-b070-61117bb6415f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_3c56f50e-3241-40fe-94b8-c2e59042e3f7" xlink:to="loc_us-gaap_PlanNameAxis_9aa52518-2eeb-449f-b070-61117bb6415f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_9aa52518-2eeb-449f-b070-61117bb6415f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_9aa52518-2eeb-449f-b070-61117bb6415f" xlink:to="loc_us-gaap_PlanNameDomain_9aa52518-2eeb-449f-b070-61117bb6415f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_3b74ce44-1a99-444a-99ae-baef90ced032" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_9aa52518-2eeb-449f-b070-61117bb6415f" xlink:to="loc_us-gaap_PlanNameDomain_3b74ce44-1a99-444a-99ae-baef90ced032" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_A2019EquityIncentivePlanMember_462a53a5-06d3-49b7-8711-488eeb8443b0" xlink:href="ncno-20201031.xsd#ncno_A2019EquityIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_3b74ce44-1a99-444a-99ae-baef90ced032" xlink:to="loc_ncno_A2019EquityIncentivePlanMember_462a53a5-06d3-49b7-8711-488eeb8443b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_EmployeeStockPurchasePlanMember_be1ec0aa-ba26-4edd-bcd4-31b481481df7" xlink:href="ncno-20201031.xsd#ncno_EmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_3b74ce44-1a99-444a-99ae-baef90ced032" xlink:to="loc_ncno_EmployeeStockPurchasePlanMember_be1ec0aa-ba26-4edd-bcd4-31b481481df7" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/StockBasedCompensationStockOptionActivityDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockBasedCompensationStockOptionActivityDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockBasedCompensationStockOptionActivityDetails" xlink:type="extended" id="i8d34b12b1abb4eec9a53e724441eb0ac_StockBasedCompensationStockOptionActivityDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/StockBasedCompensationRSUsActivityDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#StockBasedCompensationRSUsActivityDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/StockBasedCompensationRSUsActivityDetails" xlink:type="extended" id="ia44147afff2d40bf81d9a928c0fe0798_StockBasedCompensationRSUsActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4edf2120-3882-41e5-947e-b6853c2282ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4edf2120-3882-41e5-947e-b6853c2282ed" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_392cc85c-5f0b-4924-b5b9-b7f3f7de4fb0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_392cc85c-5f0b-4924-b5b9-b7f3f7de4fb0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_206a423e-8f41-44df-9987-bb08b4339816" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_206a423e-8f41-44df-9987-bb08b4339816" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_6a7abd9c-8d85-4dbb-95c4-35e293639354" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_6a7abd9c-8d85-4dbb-95c4-35e293639354" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_f1ce9d45-aff2-43a4-a299-26a8f122dbdf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_929d5941-0756-450a-aac6-70518d2b972e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_f1ce9d45-aff2-43a4-a299-26a8f122dbdf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_f95df383-539b-42c4-be3c-5e4ec056b361" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4edf2120-3882-41e5-947e-b6853c2282ed" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_55bd5cd5-7570-4c41-ad76-810ad199afc5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_55bd5cd5-7570-4c41-ad76-810ad199afc5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_967f5f80-9288-47c3-95ce-08416f61e996" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_967f5f80-9288-47c3-95ce-08416f61e996" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_6e42def2-c8e2-4bdc-91d4-8b5c046bd55a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_6e42def2-c8e2-4bdc-91d4-8b5c046bd55a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_9879fbc9-ce59-4c21-bba1-bd5765e4274b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_25bd8a00-f2a5-4670-a97a-ff69b2a9463f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_9879fbc9-ce59-4c21-bba1-bd5765e4274b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_2943457a-a262-4d3f-b083-cc88cb4e6e2a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_15e0bdd7-dded-4473-a74d-f2efc06ec002" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4edf2120-3882-41e5-947e-b6853c2282ed" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_15e0bdd7-dded-4473-a74d-f2efc06ec002" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_8f2ceaf1-8632-4a79-91e2-6479cb461d8b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_15e0bdd7-dded-4473-a74d-f2efc06ec002" xlink:to="loc_us-gaap_AwardTypeAxis_8f2ceaf1-8632-4a79-91e2-6479cb461d8b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8f2ceaf1-8632-4a79-91e2-6479cb461d8b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_8f2ceaf1-8632-4a79-91e2-6479cb461d8b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8f2ceaf1-8632-4a79-91e2-6479cb461d8b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8585722c-c6d4-4786-852f-42066451794e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_8f2ceaf1-8632-4a79-91e2-6479cb461d8b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8585722c-c6d4-4786-852f-42066451794e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_b21d48c5-a0ca-4e6a-a812-680e65b86dd5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8585722c-c6d4-4786-852f-42066451794e" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_b21d48c5-a0ca-4e6a-a812-680e65b86dd5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/CommitmentandContingencies" xlink:type="simple" xlink:href="ncno-20201031.xsd#CommitmentandContingencies"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/CommitmentandContingencies" xlink:type="extended" id="i9eb7f7bf15924246b65ef926422deab5_CommitmentandContingencies"/>
  <link:roleRef roleURI="http://www.ncino.com/role/CommitmentandContingenciesDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#CommitmentandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/CommitmentandContingenciesDetails" xlink:type="extended" id="ib3fef8a170f24f9c88bbded4448931ad_CommitmentandContingenciesDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/RelatedPartyTransactions" xlink:type="simple" xlink:href="ncno-20201031.xsd#RelatedPartyTransactions"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RelatedPartyTransactions" xlink:type="extended" id="i93a97384fae74ac8891d87d62bf5731b_RelatedPartyTransactions"/>
  <link:roleRef roleURI="http://www.ncino.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#RelatedPartyTransactionsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/RelatedPartyTransactionsDetails" xlink:type="extended" id="i2d32395a61a44090808cfa4b4daacf7a_RelatedPartyTransactionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_RelatedPartyNonCancellableAgreementRenewalTerm_12cc38cd-9ca2-4801-90b5-94dd3b198af4" xlink:href="ncno-20201031.xsd#ncno_RelatedPartyNonCancellableAgreementRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_ncno_RelatedPartyNonCancellableAgreementRenewalTerm_12cc38cd-9ca2-4801-90b5-94dd3b198af4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty_fd682555-97e2-4bbd-91bb-2da08c725bc8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty_fd682555-97e2-4bbd-91bb-2da08c725bc8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DueToRelatedPartiesCurrent_ff8b6f03-f77a-4ae2-8102-a19e8211bb44" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DueToRelatedPartiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_DueToRelatedPartiesCurrent_ff8b6f03-f77a-4ae2-8102-a19e8211bb44" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_NumberOfAffiliatedEntities_fad02784-53d8-45a7-847f-5cb102e06788" xlink:href="ncno-20201031.xsd#ncno_NumberOfAffiliatedEntities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_ncno_NumberOfAffiliatedEntities_fad02784-53d8-45a7-847f-5cb102e06788" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromRelatedParties_2db323f0-38a7-4c36-a4c6-ae93d7681dcd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_RevenueFromRelatedParties_2db323f0-38a7-4c36-a4c6-ae93d7681dcd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_e0da6af6-e440-4c0a-be01-09cd0499363b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_e0da6af6-e440-4c0a-be01-09cd0499363b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableRelatedParties_44c4ec07-38ea-4e96-b93c-14c84a511e65" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_AccountsReceivableRelatedParties_44c4ec07-38ea-4e96-b93c-14c84a511e65" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeRelatedParty_e6039212-757e-408e-86c2-3c667595257b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestIncomeRelatedParty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_InterestIncomeRelatedParty_e6039212-757e-408e-86c2-3c667595257b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_RelatedPartyTransactionTermOfAgreement_f0fba873-a4f3-48f6-abc4-a806a6502238" xlink:href="ncno-20201031.xsd#ncno_RelatedPartyTransactionTermOfAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_ncno_RelatedPartyTransactionTermOfAgreement_f0fba873-a4f3-48f6-abc4-a806a6502238" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_RelatedPartyTransactionTermOfAgreementExtensionPeriod_f8a1ea52-533a-4c8d-8e0d-7a1830b089aa" xlink:href="ncno-20201031.xsd#ncno_RelatedPartyTransactionTermOfAgreementExtensionPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_ncno_RelatedPartyTransactionTermOfAgreementExtensionPeriod_f8a1ea52-533a-4c8d-8e0d-7a1830b089aa" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_49a754e5-20c4-4734-8b55-b8189e57e5ae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_49a754e5-20c4-4734-8b55-b8189e57e5ae" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20becf39-0193-49e5-a2e9-763b54a45dbf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_e6a03867-5e12-452e-9450-eabf3e051d4b" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20becf39-0193-49e5-a2e9-763b54a45dbf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_e5c15b41-b744-4574-9299-3a3b8af08122" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20becf39-0193-49e5-a2e9-763b54a45dbf" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_e5c15b41-b744-4574-9299-3a3b8af08122" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_e5c15b41-b744-4574-9299-3a3b8af08122_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_e5c15b41-b744-4574-9299-3a3b8af08122" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_e5c15b41-b744-4574-9299-3a3b8af08122_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_faf9507d-e842-4f69-a684-42bec306b7dd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_e5c15b41-b744-4574-9299-3a3b8af08122" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_faf9507d-e842-4f69-a684-42bec306b7dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpensesAndOtherCurrentAssetsMember_2cf127e4-1b8e-41de-be3b-55a2ba3b990c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PrepaidExpensesAndOtherCurrentAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_faf9507d-e842-4f69-a684-42bec306b7dd" xlink:to="loc_us-gaap_PrepaidExpensesAndOtherCurrentAssetsMember_2cf127e4-1b8e-41de-be3b-55a2ba3b990c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableMember_e3fd6de5-c358-4746-9a0e-6d4c45456a56" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_faf9507d-e842-4f69-a684-42bec306b7dd" xlink:to="loc_us-gaap_AccountsPayableMember_e3fd6de5-c358-4746-9a0e-6d4c45456a56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_737c4de7-989e-4702-8f3c-a7e17dfece82" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20becf39-0193-49e5-a2e9-763b54a45dbf" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_737c4de7-989e-4702-8f3c-a7e17dfece82" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_737c4de7-989e-4702-8f3c-a7e17dfece82_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_737c4de7-989e-4702-8f3c-a7e17dfece82" xlink:to="loc_us-gaap_RelatedPartyDomain_737c4de7-989e-4702-8f3c-a7e17dfece82_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_f29566f2-1e0f-4838-929d-21d2e8c57c2d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_737c4de7-989e-4702-8f3c-a7e17dfece82" xlink:to="loc_us-gaap_RelatedPartyDomain_f29566f2-1e0f-4838-929d-21d2e8c57c2d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_23b2eec7-cc11-4023-bb70-825d32f16a55" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_f29566f2-1e0f-4838-929d-21d2e8c57c2d" xlink:to="loc_srt_AffiliatedEntityMember_23b2eec7-cc11-4023-bb70-825d32f16a55" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_5e024112-512a-43b1-a4cd-a13dbcd9baf1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20becf39-0193-49e5-a2e9-763b54a45dbf" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_5e024112-512a-43b1-a4cd-a13dbcd9baf1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_5e024112-512a-43b1-a4cd-a13dbcd9baf1_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_5e024112-512a-43b1-a4cd-a13dbcd9baf1" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_5e024112-512a-43b1-a4cd-a13dbcd9baf1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_5e024112-512a-43b1-a4cd-a13dbcd9baf1" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_AgreementForPurchaseOfServiceMember_0f287882-bf70-47e5-b6b1-eaf6064be31d" xlink:href="ncno-20201031.xsd#ncno_AgreementForPurchaseOfServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:to="loc_ncno_AgreementForPurchaseOfServiceMember_0f287882-bf70-47e5-b6b1-eaf6064be31d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_TransactionsWithCertainEquityHoldersMember_9ba48506-5f74-4909-a5c6-704aebcdd115" xlink:href="ncno-20201031.xsd#ncno_TransactionsWithCertainEquityHoldersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:to="loc_ncno_TransactionsWithCertainEquityHoldersMember_9ba48506-5f74-4909-a5c6-704aebcdd115" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_BankingRelationshipMember_71b06b95-16dd-4c96-ae98-a48d7bba786d" xlink:href="ncno-20201031.xsd#ncno_BankingRelationshipMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:to="loc_ncno_BankingRelationshipMember_71b06b95-16dd-4c96-ae98-a48d7bba786d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FundSpendingAgreementMember_a0ab96d3-6c3d-4371-a3de-4fe4d0a7fa41" xlink:href="ncno-20201031.xsd#ncno_FundSpendingAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_f80d9df6-9e8e-40b3-b271-60569180fadb" xlink:to="loc_ncno_FundSpendingAgreementMember_a0ab96d3-6c3d-4371-a3de-4fe4d0a7fa41" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/BasicandDilutedLossperShare" xlink:type="simple" xlink:href="ncno-20201031.xsd#BasicandDilutedLossperShare"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BasicandDilutedLossperShare" xlink:type="extended" id="i939d9361bb1a4234b8f283995913ba26_BasicandDilutedLossperShare"/>
  <link:roleRef roleURI="http://www.ncino.com/role/BasicandDilutedLossperShareTables" xlink:type="simple" xlink:href="ncno-20201031.xsd#BasicandDilutedLossperShareTables"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BasicandDilutedLossperShareTables" xlink:type="extended" id="i29027c2844f34185a8d8c530f036e1a8_BasicandDilutedLossperShareTables"/>
  <link:roleRef roleURI="http://www.ncino.com/role/BasicandDilutedLossperShareComponentsofBasicandDilutedLossPerShareDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BasicandDilutedLossperShareComponentsofBasicandDilutedLossPerShareDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BasicandDilutedLossperShareComponentsofBasicandDilutedLossPerShareDetails" xlink:type="extended" id="icb460b804df842c280a9aa7d5ec43e60_BasicandDilutedLossperShareComponentsofBasicandDilutedLossPerShareDetails"/>
  <link:roleRef roleURI="http://www.ncino.com/role/BasicandDilutedLossperShareNarrativeDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BasicandDilutedLossperShareNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BasicandDilutedLossperShareNarrativeDetails" xlink:type="extended" id="i2a887c39957740abab26d0ad3ee19281_BasicandDilutedLossperShareNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_68d1313e-b638-49a2-a89a-cd7f2b46076d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedNumberOfShares_8a04e98e-b4ab-47ad-b298-d9f1efc8d50e" xlink:href="ncno-20201031.xsd#ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_68d1313e-b638-49a2-a89a-cd7f2b46076d" xlink:to="loc_ncno_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedNumberOfShares_8a04e98e-b4ab-47ad-b298-d9f1efc8d50e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_2361ab94-6036-4330-9f7e-11cfcd0bf232" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_68d1313e-b638-49a2-a89a-cd7f2b46076d" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_2361ab94-6036-4330-9f7e-11cfcd0bf232" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_ba622b3e-bb3d-4729-9643-e7eab1949cf7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_68d1313e-b638-49a2-a89a-cd7f2b46076d" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_ba622b3e-bb3d-4729-9643-e7eab1949cf7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_2d937970-b8bc-4e79-b330-bc104088f07f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_ba622b3e-bb3d-4729-9643-e7eab1949cf7" xlink:to="loc_us-gaap_AwardTypeAxis_2d937970-b8bc-4e79-b330-bc104088f07f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2d937970-b8bc-4e79-b330-bc104088f07f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_2d937970-b8bc-4e79-b330-bc104088f07f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2d937970-b8bc-4e79-b330-bc104088f07f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_901a4099-747c-4d5e-b650-1cd839fe9b27" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_2d937970-b8bc-4e79-b330-bc104088f07f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_901a4099-747c-4d5e-b650-1cd839fe9b27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_00dc0eed-52f5-4632-ab5b-3ab4a6ac9fed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_901a4099-747c-4d5e-b650-1cd839fe9b27" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_00dc0eed-52f5-4632-ab5b-3ab4a6ac9fed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationByTypeAxis_5f725348-2262-4da5-8329-e55b48615905" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_ba622b3e-bb3d-4729-9643-e7eab1949cf7" xlink:to="loc_us-gaap_ContingentConsiderationByTypeAxis_5f725348-2262-4da5-8329-e55b48615905" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_5f725348-2262-4da5-8329-e55b48615905_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_5f725348-2262-4da5-8329-e55b48615905" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_5f725348-2262-4da5-8329-e55b48615905_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_74ccb48a-8b31-406a-a9f9-efe911a490c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_5f725348-2262-4da5-8329-e55b48615905" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_74ccb48a-8b31-406a-a9f9-efe911a490c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_FirstInitialTrancheEarnOutMember_77c053ab-e409-4f7b-9512-665081de4c06" xlink:href="ncno-20201031.xsd#ncno_FirstInitialTrancheEarnOutMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_74ccb48a-8b31-406a-a9f9-efe911a490c6" xlink:to="loc_ncno_FirstInitialTrancheEarnOutMember_77c053ab-e409-4f7b-9512-665081de4c06" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/BasicandDilutedLossperShareWeightedAverageNumberofSharesExcludedFromComputationofEPSDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#BasicandDilutedLossperShareWeightedAverageNumberofSharesExcludedFromComputationofEPSDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/BasicandDilutedLossperShareWeightedAverageNumberofSharesExcludedFromComputationofEPSDetails" xlink:type="extended" id="ib88975da8a2b4f30950dc3fd3cb2a1a0_BasicandDilutedLossperShareWeightedAverageNumberofSharesExcludedFromComputationofEPSDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_07042997-1a68-45ff-bcb5-8e92ce4763d9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_87c260ab-89b6-4e9f-98ee-37f9b074c3b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_07042997-1a68-45ff-bcb5-8e92ce4763d9" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_87c260ab-89b6-4e9f-98ee-37f9b074c3b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_f617dbd6-c374-499f-98b0-cb7713fe998c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_07042997-1a68-45ff-bcb5-8e92ce4763d9" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_f617dbd6-c374-499f-98b0-cb7713fe998c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_f617dbd6-c374-499f-98b0-cb7713fe998c" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_eda9e738-3190-4c53-abec-dcc2155d5485" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9aad2e0d-77ff-4cbb-8c3f-f58e634c29a5" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_eda9e738-3190-4c53-abec-dcc2155d5485" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_c680d88c-9037-4421-938b-a4b35f22cf94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_eda9e738-3190-4c53-abec-dcc2155d5485" xlink:to="loc_us-gaap_EmployeeStockOptionMember_c680d88c-9037-4421-938b-a4b35f22cf94" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_e21f8253-7899-430f-9364-923ee363bf1f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_eda9e738-3190-4c53-abec-dcc2155d5485" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_e21f8253-7899-430f-9364-923ee363bf1f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.ncino.com/role/SubsequentEvents" xlink:type="simple" xlink:href="ncno-20201031.xsd#SubsequentEvents"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SubsequentEvents" xlink:type="extended" id="i2184dcad35f24683a2d65672da704d95_SubsequentEvents"/>
  <link:roleRef roleURI="http://www.ncino.com/role/SubsequentEventsDetails" xlink:type="simple" xlink:href="ncno-20201031.xsd#SubsequentEventsDetails"/>
  <link:definitionLink xlink:role="http://www.ncino.com/role/SubsequentEventsDetails" xlink:type="extended" id="i86ade0d8819b46369ff19af9ee1fd9bf_SubsequentEventsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_LesseeOperatingLeaseOfficeSpace_94008010-bff8-4a70-ba6f-cf0d204a79ab" xlink:href="ncno-20201031.xsd#ncno_LesseeOperatingLeaseOfficeSpace"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_LesseeOperatingLeaseOfficeSpace_94008010-bff8-4a70-ba6f-cf0d204a79ab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseTermOfContract_bd70419f-9ff4-4bf1-b142-fcdcf6c7584e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_us-gaap_LesseeOperatingLeaseTermOfContract_bd70419f-9ff4-4bf1-b142-fcdcf6c7584e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_LesseeOperatingLeaseNumberOfRenewalTerms_e04e8850-9e0f-42ba-b4df-5d32bde4ad93" xlink:href="ncno-20201031.xsd#ncno_LesseeOperatingLeaseNumberOfRenewalTerms"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_LesseeOperatingLeaseNumberOfRenewalTerms_e04e8850-9e0f-42ba-b4df-5d32bde4ad93" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_48cf16fe-e9eb-4bc7-8f47-e1dc66c9f102" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_48cf16fe-e9eb-4bc7-8f47-e1dc66c9f102" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_LesseeOperatingLeaseMonthlyRentPayments_12aec79b-cbca-426f-9aa5-538736445d18" xlink:href="ncno-20201031.xsd#ncno_LesseeOperatingLeaseMonthlyRentPayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_LesseeOperatingLeaseMonthlyRentPayments_12aec79b-cbca-426f-9aa5-538736445d18" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_LesseeOperatingLeaseAnnualIncreaseInRentPercent_b78d30e5-5dee-430b-a4bb-275fd8ff771b" xlink:href="ncno-20201031.xsd#ncno_LesseeOperatingLeaseAnnualIncreaseInRentPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_LesseeOperatingLeaseAnnualIncreaseInRentPercent_b78d30e5-5dee-430b-a4bb-275fd8ff771b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_PurchasePricePurchaseOptionExercised_ef7e3398-58c7-4614-8351-2c62b4de75a3" xlink:href="ncno-20201031.xsd#ncno_PurchasePricePurchaseOptionExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_PurchasePricePurchaseOptionExercised_ef7e3398-58c7-4614-8351-2c62b4de75a3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ncno_LesseeOperatingLeaseParkingDeck_cde362e5-2cb3-4392-9059-2f6250b69067" xlink:href="ncno-20201031.xsd#ncno_LesseeOperatingLeaseParkingDeck"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_ncno_LesseeOperatingLeaseParkingDeck_cde362e5-2cb3-4392-9059-2f6250b69067" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_9ab23c24-eaf1-4d86-bd70-e48e05d743c8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_8cd36977-6c9f-4927-baf2-e24f66eef812" xlink:to="loc_us-gaap_SubsequentEventTable_9ab23c24-eaf1-4d86-bd70-e48e05d743c8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_a11063a8-1d70-4797-8557-14a47d34416f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_9ab23c24-eaf1-4d86-bd70-e48e05d743c8" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_a11063a8-1d70-4797-8557-14a47d34416f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_a11063a8-1d70-4797-8557-14a47d34416f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_a11063a8-1d70-4797-8557-14a47d34416f" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_a11063a8-1d70-4797-8557-14a47d34416f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_a731df92-9e1d-4b5a-afb5-721c42afa20a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_a11063a8-1d70-4797-8557-14a47d34416f" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_a731df92-9e1d-4b5a-afb5-721c42afa20a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_6f5c7d64-e395-4b29-bd24-346b263f694e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_a731df92-9e1d-4b5a-afb5-721c42afa20a" xlink:to="loc_us-gaap_SubsequentEventMember_6f5c7d64-e395-4b29-bd24-346b263f694e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_9ab23c24-eaf1-4d86-bd70-e48e05d743c8" xlink:to="loc_srt_StatementScenarioAxis_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6" xlink:to="loc_srt_ScenarioUnspecifiedDomain_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_32d89bc4-ca55-42b3-831e-492e104b0f81" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_e4f9b1ec-97e0-4e45-92e1-3c45253a9ec6" xlink:to="loc_srt_ScenarioUnspecifiedDomain_32d89bc4-ca55-42b3-831e-492e104b0f81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_20913c01-1710-4725-8f7f-97ab986580c5" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_32d89bc4-ca55-42b3-831e-492e104b0f81" xlink:to="loc_srt_ScenarioForecastMember_20913c01-1710-4725-8f7f-97ab986580c5" xlink:type="arc" order="0"/>
  </link:definitionLink>
</link:linkbase>
